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NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 145-302)

Details for Product anti-NFKBIA Antibody No. ABIN968113, Supplier: Log in to see
Antigen
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Alternatives
anti-Human NFKBIA antibody for Western Blotting
Epitope
AA 145-302
67
53
48
37
24
23
22
22
19
15
14
13
13
13
7
5
5
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
473
213
176
35
30
22
8
6
2
1
Host
Mouse
331
148
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
This NFKBIA antibody is un-conjugated
18
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
362
183
143
86
85
77
73
34
32
19
6
5
4
3
2
1
1
1
1
1
1
Supplier
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Immunogen Human IkappaBalpha aa. 145-302
Clone 25-IkBa-MAD
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Ikappa B alpha (NFKBIA Antibody Abstract)
Background NF-kappaB is a transcription factor that regulates many cytokine and Ig genes involved in immune response, inflammation, and viral infections. NF-kappaB is induced by cytokines and lipopolysaccharide (LPS), but is inhibited by glucocorticoids. IkappaBalpha/MAD-3 inhibits the DNA-binding activity of NF-kappaB and related transcription factors. In THP-1 monocytic-like cells, LPS induces the phosphorylation and inactivation of IkappaBalpha, which results in the activation of NF-kappaB. Therefore, phosphorylation of IkappaBalpha may induce the dissociation of the IkappaBalpha/NF-kappaB complex.
Synonyms: MAD-3
Molecular Weight 38 kDa
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 145-302) (ABIN968113) Western blot of IkappaBalpha on human endothelial lysate. Lane 1: 1:500, lane 2: 1:10...
 image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 145-302) (ABIN968113) Immunofluroescent staining of MCF7 cells with anti-IkappaBalpha antibody.
 image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 145-302) (ABIN968113) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 145-302) antibody (Image 3)
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (AA 145-302) (ABIN968113) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 145-302) antibody (Image 4)
Product cited in: Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000 (PubMed).

Auphan, DiDonato, Rosette, Helmberg, Karin: "Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis." in: Science (New York, N.Y.), Vol. 270, Issue 5234, pp. 286-90, 1995 (PubMed).

Traenckner, Pahl, Henkel, Schmidt, Wilk, Baeuerle: "Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli." in: The EMBO journal, Vol. 14, Issue 12, pp. 2876-83, 1995 (PubMed).

Cordle, Donald, Read, Hawiger: "Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-kappa B proteins in human monocytic THP-1 cells." in: The Journal of biological chemistry, Vol. 268, Issue 16, pp. 11803-10, 1993 (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

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