Enabled Homolog (Drosophila) (ENAH) (AA 415-541) antibody

Antigen: Enabled Homolog (Drosophila) (ENAH)

Synonyms: NP94, LSFR1, ZNF356, 0610038H21Rik, 2900056O04Rik, CIZ1, id:ibd2798, si:ch211-215p14.1

Binding Site: AA 415-541

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN968115

Additional Information

Alternative name: Mena

Immunogen: Mouse Mena

Cross-Reactivity: Human, Rat (Rattus), Chicken

Format: Liquid

Isotype: IgA

Clone: 21

Description: Abl and Disabled (Dab) are essential for correct axonal connections during Drosophila development. Mutations in Enabled (Ena) rescue the genetic defects caused by mutated Abl and Dab. Mena (Mammalian Ena) is a protein with significant homology to Eva, VASP, and WASP proteins. The murine Mena gene predicts a protein of 541 amino acids. However, two additional exons that could introduce 246 amino acids onto the C-terminal region have been identified. Homology searches identified two EVH domains (Ena-Vasp homology), one near each terminus of Mena. Antibodies to Mena detect 80 kDa, 88 kDa, and 140 kDa protein with the 80 kDa and 88 kDa proteins being widely expressed and the 140 kDa protein reportedly mainly found in brain tissue. Like VASP, Mena is localized to focal contacts and, when ectopically expressed, induces the formation of F-actin outgrowths in fibroblasts. This antibody is routinely tested by Western blot analysis.
Synonyms: Mammalian Ena (Enabled)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 140, 88, 80 kDa

Comments:

Related Products: ABIN968552, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Mena on a SW13 cell lysate (Human adrenal gland carcinoma, ATCC CCL-105). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Mena antibody.

Immunofluorescence staining of human endothelial cells.

Immunohistochemical staining on rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment (40X magnification).

Publications

Product: Gertler, Niebuhr, Reinhard et al.: "Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics." in: Cell, Vol. 87, Issue 2, pp. 227-39, 1996 (PubMed).

Drees, Friederich, Fradelizi et al.: "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22503-11, 2000 (PubMed).

García Arguinzonis, Galler, Walter et al.: "Increased spreading, Rac/p21-activated kinase (PAK) activity, and compromised cell motility in cells deficient in vasodilator-stimulated phosphoprotein (VASP)." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45604-10, 2002 (PubMed).

Howe, Hogan, Juliano: "Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38121-6, 2002 (PubMed).