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Minichromosome Maintenance Complex Component 2 (MCM2) (AA 725-888) antibody

Details for Product No. ABIN968121, Supplier: Log in to see
Antigen
  • AA959861
  • AW476101
  • BM28
  • bm28
  • cb737
  • CCNL1
  • ccnl1
  • cdc19
  • CDCL1
  • cdcl1
  • CG7538
  • chunp6905
  • D3S3194
  • dMCM2
  • Dmel\\CG7538
  • DmeMCM2
  • DmMcm2
  • DmMCM2
  • l(3)rL074
  • MCM2
  • McM2
  • MCM2-7
  • MCM2_DROME
  • MCM7
  • Mcmd2
  • MITOTIN
  • mitotin
  • mKIAA0030
  • PCR3
  • XMcm2
Alternatives
anti-Human Minichromosome Maintenance Complex Component 2 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 725-888
41
16
16
15
13
12
10
10
9
9
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
244
83
46
5
4
3
2
2
1
1
1
Host
Mouse
194
72
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
9
9
9
5
5
5
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
217
92
86
78
50
32
23
23
11
10
8
5
3
3
2
1
1
Supplier
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Immunogen Human BM28 aa. 725-888
Clone 46-BM28
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name BM28 (MCM2 Antibody Abstract)
Background BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.
Molecular Weight 125 kDa
Pathways DNA Damage Repair
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 725-888) antibody (ABIN968121) Western blot analysis of BM28 on human endothelial cell lysate. Lane 1: 1:500, lane 2...
Immunofluorescence (IF) image for anti-Minichromosome Maintenance Complex Component 2 (MCM2) (AA 725-888) antibody (ABIN968121) Immunofluorescent staining of Human Endothelial cells.
Product cited in: Cook, Park, Burke, Leone, DeGregori, Engel, Nevins: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, 2002 (PubMed).

Shreeram, Sparks, Lane, Blow: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, 2002 (PubMed).

Todorov, Pepperkok, Philipova, Kearsey, Ansorge, Werner: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, 1994 (PubMed).

Background publications Ishimi, Ichinose, Omori, Sato, Kimura: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, 1996 (PubMed).

Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, 1995 (PubMed).