NFAT1 antibody (AA 29-181)

Catalog No. ABIN968123

Product Details anti-NFAT1 Antibody

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Binding Specificity: AA 29-181

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NFAT-1 aa. 29-181

Clone: 1-NFAT

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968537, ABIN968619

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-NFAT1 antibody (ABIN968123)

Grader-Beck, van Puijenbroek, Nadler, Boussiotis: "cAMP inhibits both Ras and Rap1 activation in primary human T lymphocytes, but only Ras inhibition correlates with blockade of cell cycle progression." in: Blood, Vol. 101, Issue 3, pp. 998-1006, (2003) (PubMed).

Kadereit, Mohammad, Miller, Woods, Listrom, McKinnon, Alali, Bos, Iacobucci, Sramkoski, Jacobberger, Laughlin: "Reduced NFAT1 protein expression in human umbilical cord blood T lymphocytes." in: Blood, Vol. 94, Issue 9, pp. 3101-7, (1999) (PubMed).

Good, Maggirwar, Harhaj, Sun: "Constitutive dephosphorylation and activation of a member of the nuclear factor of activated T cells, NF-AT1, in Tax-expressing and type I human T-cell leukemia virus-infected human T cells." in: The Journal of biological chemistry, Vol. 272, Issue 3, pp. 1425-8, (1997) (PubMed).

Boise, Petryniak, Mao, June, Wang, Lindsten, Bravo, Kovary, Leiden, Thompson: "The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB." in: Molecular and cellular biology, Vol. 13, Issue 3, pp. 1911-9, (1993) (PubMed).

McCaffrey, Luo, Kerppola, Jain, Badalian, Ho, Burgeon, Lane, Lambert, Curran: "Isolation of the cyclosporin-sensitive T cell transcription factor NFATp." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 750-4, (1993) (PubMed).

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFAT-1 (NFAT1 Products)

Synonyms: NFATC2 antibody, AI607462 antibody, NF-ATc2 antibody, NFAT1 antibody, NFAT1-D antibody, Nfatp antibody, NFATP antibody, nuclear factor of activated T-cells 2 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 antibody, NFATC2 antibody, nfatc2 antibody, Nfatc2 antibody

Background: T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.
Synonyms: NF-ATc2, NFATP, KIAA0611

Molecular Weight: 97-135 kDa

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling