A Kinase (PRKA) Anchor Protein 11 (AKAP11) (AA 11-173) antibody
|Synonyms||Gm80, PRKA11, AKAP220, mKIAA0629, 6330501D17Rik, Akap, AKAP11, prka11, akap220, FLJ11304, KIAA0629, DKFZp781I12161|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|3 references available|
|Price||Product not available in this region.|
|Description||The type II cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain this localization of type II PKAs, the regulatory subunit (RII) interacts with RII-anchoring proteins. For instance, attachment of type II PKA to the cytoskeleton occurs through the binding of RII to microtubule associated-protein 2. In brain, several proteins have been identified as PKA type II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP220 is a protein of 1129 amino acids with a PKARII binding site and a CRL motif at the COOH terminus, characteristic of peroxisomal proteins. AKAP220 mRNA is expressed ubiquitously with the highest levels in testis and brain. Immunolocalization studies confirmed the peroxisomal distribution of both AKAP220 and PKARII in a cell line derived from rat Sertoli cells.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||220 kDa|
Related Products: ABIN968545, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of AKAP220 on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:2000 dilution of anti-AKAP220. Immunofluroescent staining of Rabbit Heart with anti-AKAP220.|
Lester, Coghlan, Nauert et al.: "Cloning and characterization of a novel A-kinase anchoring protein. AKAP 220, association with testicular peroxisomes." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9460-5, 1996 (PubMed).
DellAcqua, Scott: "Protein kinase A anchoring." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, 1997 (PubMed).
Tanji, Yamamoto, Yorioka et al.: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, 2002 (PubMed).
|Reactivities||Human (5), Mouse (Murine) (3), Rat (Rattus) (3), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||ELISA (5), Western Blotting (WB) (4), Immunofluorescence (IF) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (1)|