anti-Amphiphysin antibody (AMPH) (AA 258-414)

Details for Product anti-AMPH Antibody No. ABIN968131
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Antigen
Synonyms Amp, CG8604, DAMP, Damp, Dmel\\CG8604, amph, dAmph, damph, AMPH, Amph, GB16263, AMPH1, Amph1, wu:fq25h04, zgc:73193
Epitope
AA 258-414
(31), (15), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(118), (53), (49), (8), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(68), (29), (22), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This Amphiphysin antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(109), (50), (43), (22), (20), (10), (7), (4), (3), (3), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 50 μg
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Immunogen Human Amphiphysin
Clone Ghr5
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Amphiphysin (AMPH Antibody Abstract)
Background Amphiphysin was originally described in chicken brain and located at the presynaptic terminal. It was also identified as the major autoantigen in Stiff-Man Syndrome (SMS), a neurological disease linked to an autoimmune response to antigens in the central nervous system. Amphiphysin interacts with several proteins important in the synaptic vesicle cycle. Amphiphysin contains a Src homology 3 (SH3) domain that binds to the C-terminal and proline-rich region of dynamin at the synapse. Microinjection of amphiphysin inhibits synaptic vesicle endocytosis. In addition, amphiphysin binds to AP-2 at the plasma membrane and to the inositol 5-phosphatase, synaptojanin. The interaction between synaptojanin and amphiphysin also appears to be mediated through amphiphysin's SH3-domain.
Molecular Weight 125 kDa
Research Area Neurology
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Amphiphysin (AMPH) (AA 258-414) antibody Western blot of Amphiphysin on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1: 20...
anti-Amphiphysin (AMPH) (AA 258-414) antibody (2) Immunohistochemical staining for Amphiphysin on a formalin-fixed paraffin-embedded se...
anti-Amphiphysin (AMPH) (AA 258-414) antibody (3) anti-Amphiphysin (AMPH) (AA 258-414) antibody (Image 3)
Product cited in: Drake, Traub: "Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28700-9, 2001 (PubMed).

Traub, Downs, Westrich et al.: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, 1999 (PubMed).

David, Solimena, De Camilli: "Autoimmunity in stiff-Man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161." in: FEBS letters, Vol. 351, Issue 1, pp. 73-9, 1994 (PubMed).

Wang, Südhof, Anderson: "The appendage domain of alpha-adaptin is a high affinity binding site for dynamin." in: The Journal of biological chemistry, Vol. 270, Issue 17, pp. 10079-83, 1995 (PubMed).

Validation Images
Catalog No. ABIN968131
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