AKAP1 antibody (AA 66-212)

Catalog No. ABIN968136

Product Details anti-AKAP1 Antibody

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Binding Specificity: AA 66-212

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human AKAP149 aa. 66-212

Clone: 6-AKAP149

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-AKAP1 antibody (ABIN968136)

Schillace, Andrews, Liberty, Davey, Carr: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, (2002) (PubMed).

Tanji, Yamamoto, Yorioka, Kohno, Kikuchi, Kikuchi: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, (2002) (PubMed).

Steen, Collas: "Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression." in: The Journal of cell biology, Vol. 153, Issue 3, pp. 621-6, (2001) (PubMed).

DellAcqua, Scott: "Protein kinase A anchoring." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, (1997) (PubMed).

Trendelenburg, Hummel, Riecken, Hanski: "Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain." in: Biochemical and biophysical research communications, Vol. 225, Issue 1, pp. 313-9, (1996) (PubMed).

Target Details for AKAP1

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Alternative Name: AKAP149 (AKAP1 Products)

Background: The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity. This antibody is routinely tested by western blot analysis.

Molecular Weight: 149 kDa

Pathways: Synaptic Membrane