Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody

Antigen: Lymphocyte-Specific Protein 1 (LSP1)

Synonyms: WP34, pp52, LSP1, p50, Lsp-1

Binding Site: AA 75-215

Clonality: Monoclonal (16)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968145

Additional Information

Alternative name: LSP-1

Immunogen: Human LSP-1

Format: Liquid

Isotype: IgG1

Clone: 16

Description: LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction. LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol. This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells. LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin. These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells. The mRNA is constitutively expressed, however, the protein is only expressed following activation of the cell. Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils. This antibody is routinely tested by western blot analysis.
Synonyms: Lymphocyte Specific Protein-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 52 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of LSP-1 on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human LSP-1 antibody.

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).

anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (Image 3)

Publications

Product: Kadiyala, McIntyre, Krensky: "Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." in: European journal of immunology, Vol. 20, Issue 11, pp. 2417-23, 1991 (PubMed).

Jongstra-Bilen, Young, Chong et al.: "Human and mouse LSP1 genes code for highly conserved phosphoproteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 1104-10, 1990 (PubMed).

Klein, Jongstra-Bilen, Ogryzlo et al.: "Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 3043-8, 1989 (PubMed).

Matsumoto, Kojima, Osawa et al.: "Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes." in: Journal of biochemistry, Vol. 117, Issue 1, pp. 222-9, 1995 (PubMed).

Howard, Li, Torres et al.: "The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." in: Blood, Vol. 83, Issue 1, pp. 231-41, 1994 (PubMed).