Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody
|Synonyms||WP34, pp52, LSP1, p50, Lsp-1|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction. LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol. This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells. LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin. These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells. The mRNA is constitutively expressed, however, the protein is only expressed following activation of the cell. Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils. This antibody is routinely tested by western blot analysis.
Synonyms: Lymphocyte Specific Protein-1
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||52 kDa|
Related Products: ABIN968533, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Kadiyala, McIntyre, Krensky: "Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." in: European journal of immunology, Vol. 20, Issue 11, pp. 2417-23, 1991 (PubMed).
Jongstra-Bilen, Young, Chong et al.: "Human and mouse LSP1 genes code for highly conserved phosphoproteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 1104-10, 1990 (PubMed).
Klein, Jongstra-Bilen, Ogryzlo et al.: "Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 3043-8, 1989 (PubMed).
Matsumoto, Kojima, Osawa et al.: "Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes." in: Journal of biochemistry, Vol. 117, Issue 1, pp. 222-9, 1995 (PubMed).
Howard, Li, Torres et al.: "The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." in: Blood, Vol. 83, Issue 1, pp. 231-41, 1994 (PubMed).
|Hosts||Rabbit (75), Goat (6), Mouse (2)|
|Reactivities||Human (83), Dog (Canine) (39), Mouse (Murine) (39), Rat (Rattus) (39), Cow (Bovine) (21), Chicken (19), Horse (Equine) (18), Cat (Feline) (1)|
|Applications||Immunofluorescence (IF) (46), Western Blotting (WB) (38), ELISA (33), Flow Cytometry (FACS) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunoprecipitation (IP) (6), Immunohistochemistry (IHC) (4), Immunoelectron Microscopy (IEM) (3)|
|Conjugates||Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3)|
|Epitopes||pSer252 (19), pSer204 (18), Internal Region (7), AA 261-275 (2), AA 37-48 (2), C-Term (2), AA 240-339 (1), Center (1), Internal Region,AA 115-144 (1), N-Term (1), Ser252 (1)|