Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody

Details for Product No. ABIN968145
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Antigen
Synonyms LSP1, Lsp-1, WP34, p50, pp52
Epitope
AA 75-215
(19), (18), (15), (7), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(97), (34), (33), (31), (19), (17)
Host
Mouse
(83), (9), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(67), (35), (34), (18), (18), (8), (6), (3), (2), (2), (1)
Pubmed 5 references available
Quantity 50 μg
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Catalog No. ABIN968145
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Immunogen Human LSP-1
Clone Ghr6
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name LSP-1
Background LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction. LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol. This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells. LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin. These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells. The mRNA is constitutively expressed, however, the protein is only expressed following activation of the cell. Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils. This antibody is routinely tested by western blot analysis.
Synonyms: Lymphocyte Specific Protein-1
Molecular Weight 52 kDa
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).
anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (2) Western blot analysis of LSP-1 on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human LSP-1 antibody.
anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (3) anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (Image 3)
Product cited in: Kadiyala, McIntyre, Krensky: "Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." in: European journal of immunology, Vol. 20, Issue 11, pp. 2417-23, 1991 (PubMed).

Jongstra-Bilen, Young, Chong et al.: "Human and mouse LSP1 genes code for highly conserved phosphoproteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 1104-10, 1990 (PubMed).

Klein, Jongstra-Bilen, Ogryzlo et al.: "Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 3043-8, 1989 (PubMed).

Matsumoto, Kojima, Osawa et al.: "Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes." in: Journal of biochemistry, Vol. 117, Issue 1, pp. 222-9, 1995 (PubMed).

Howard, Li, Torres et al.: "The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." in: Blood, Vol. 83, Issue 1, pp. 231-41, 1994 (PubMed).

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