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Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody

Details for Product No. ABIN968145, Supplier: Login to see
Antigen
  • LSP1
  • Lsp-1
  • WP34
  • p50
  • pp52
Epitope
AA 75-215
30
16
15
13
13
13
13
8
6
6
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Human
139
38
37
6
6
2
Host
Mouse
100
30
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
4
4
4
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
104
58
33
23
21
15
4
4
3
2
Supplier
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Immunogen Human LSP-1 aa. 75-215
Clone 16-LSP
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name LSP-1 (LSP1 Antibody Abstract)
Background LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction. LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol. This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells. LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin. These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells. The mRNA is constitutively expressed, however, the protein is only expressed following activation of the cell. Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils. This antibody is routinely tested by western blot analysis.
Synonyms: Lymphocyte Specific Protein-1
Molecular Weight 52 kDa
Pathways
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (ABIN968145) Western blot analysis of LSP-1 on a A431 cell lysate (Human epithelial carcinoma, ATC...
Immunofluorescence (IF) image for anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (ABIN968145) Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).
 image for anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (ABIN968145) anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (Image 3)
Western Blotting (WB) image for anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (ABIN968145) anti-Lymphocyte-Specific Protein 1 (LSP1) (AA 75-215) antibody (Image 4)
Product cited in: Howard, Li, Torres et al.: "The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." in: Blood, Vol. 83, Issue 1, pp. 231-41, 1994 (PubMed).

Matsumoto, Kojima, Osawa et al.: "Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes." in: Journal of biochemistry, Vol. 117, Issue 1, pp. 222-9, 1995 (PubMed).

Klein, Jongstra-Bilen, Ogryzlo et al.: "Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 3043-8, 1989 (PubMed).

Jongstra-Bilen, Young, Chong et al.: "Human and mouse LSP1 genes code for highly conserved phosphoproteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 1104-10, 1990 (PubMed).

Kadiyala, McIntyre, Krensky: "Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." in: European journal of immunology, Vol. 20, Issue 11, pp. 2417-23, 1991 (PubMed).