Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody

Antigen: Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)

Synonyms: SLP76, SLP-76

Binding Site: AA 369-520

Clonality: Monoclonal (8)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968146

Additional Information

Alternative name: SLP-76

Immunogen: Human SLP-76

Format: Liquid

Isotype: IgG1

Clone: 8

Description: SLP-76 (SH2 domain-containing Leukocyte Protein) is a tyrosine phosphoprotein that is involved in the TCR mediated intracellular signaling pathway. It may be involved in the signaling pathways of peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the amino-terminus three 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Grb2, PLC-gamma, SLAP-130, and Vav-all of which are part of the signaling cascade in T cells. ZAP-70 appears to phosphorylate SLP-76, allowing phosphorylated Vav to bind and, together the SLP-76/Vav complex, cooperatively stimulates NF-AT and IL-2 gene activation. Overexpression of SLP-76 has been reported to increase the TCR-mediated transcription of the IL-2 gene. Furthermore, SHP-1 associates with SLP-76 in normal splenic B cells which suggests a role for SLP-76 in BCR signaling.
Synonyms: SH2 domain-containing Leukocyte Protein

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 76 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of SLP-76 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human SLP-76 antibody.

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).

anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody (Image 3)

Publications

Product: Jackman, Motto, Sun et al.: "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7029-32, 1995 (PubMed).

Wu, Motto, Koretzky et al.: "Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation." in: Immunity, Vol. 4, Issue 6, pp. 593-602, 1996 (PubMed).

Tuosto, Michel, Acuto: "p95vav associates with tyrosine-phosphorylated SLP-76 in antigen-stimulated T cells." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1161-6, 1997 (PubMed).

Zhang, Trible, Zhu et al.: "Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23355-61, 2000 (PubMed).

Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, 2000 (PubMed).