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Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody

Details for Product No. ABIN968146, Supplier: Login to see
Antigen
  • AI323664
  • BB161688
  • SLP-76
  • SLP76
  • twm
  • Slp76
Alternatives
anti-Human Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 369-520
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19
15
6
4
4
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
133
56
42
10
4
3
1
1
1
Host
Mouse
79
38
21
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
109
39
28
23
15
11
7
7
5
4
2
2
1
Supplier
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Immunogen Human SLP-76 aa. 369-520
Clone 8-SLP
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SLP-76 (LCP2 Antibody Abstract)
Background SLP-76 (SH2 domain-containing Leukocyte Protein) is a tyrosine phosphoprotein that is involved in the TCR mediated intracellular signaling pathway. It may be involved in the signaling pathways of peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the amino-terminus three 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Grb2, PLC-gamma, SLAP-130, and Vav-all of which are part of the signaling cascade in T cells. ZAP-70 appears to phosphorylate SLP-76, allowing phosphorylated Vav to bind and, together the SLP-76/Vav complex, cooperatively stimulates NF-AT and IL-2 gene activation. Overexpression of SLP-76 has been reported to increase the TCR-mediated transcription of the IL-2 gene. Furthermore, SHP-1 associates with SLP-76 in normal splenic B cells which suggests a role for SLP-76 in BCR signaling.
Synonyms: SH2 domain-containing Leukocyte Protein
Molecular Weight 76 kDa
Research Area Signaling
Pathways Fc-epsilon Receptor Signaling Pathway
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody (ABIN968146) Western blot analysis of SLP-76 on a Jurkat cell lysate (Human T-cell leukemia, ATCC ...
Immunofluorescence (IF) image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody (ABIN968146) Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).
 image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody (ABIN968146) anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 369-520) antibody (Image 3)
Product cited in: Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, 2000 (PubMed).

Zhang, Trible, Zhu et al.: "Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23355-61, 2000 (PubMed).

Tuosto, Michel, Acuto: "p95vav associates with tyrosine-phosphorylated SLP-76 in antigen-stimulated T cells." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1161-6, 1997 (PubMed).

Wu, Motto, Koretzky et al.: "Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation." in: Immunity, Vol. 4, Issue 6, pp. 593-602, 1996 (PubMed).

Jackman, Motto, Sun et al.: "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7029-32, 1995 (PubMed).