RAN Binding Protein 1 (RANBP1) (AA 28-163) antibody

Antigen: RAN Binding Protein 1 (RANBP1)

Synonyms: HTF9A, MGC88701, Htf9a, MGC64314, ranbp2, MGC76028, RANBP1, MGC127635, MZN1.4, MZN1_4, RAN BINDING PROTEIN 1, zeh1094, zgc:85649, hm:zeh1094, wu:fa96a09, wu:fj39g07

Binding Site: AA 28-163

Clonality: Monoclonal (35)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968158

Additional Information

Alternative name: RanBP1

Immunogen: Human RanBP1

Format: Liquid

Isotype: IgG2a

Clone: 35

Description: Ran is a highly conserved GTPase that is ubiquitously expressed. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis, and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 29kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1. In addition, RanBP1 appears to regulate the activity of Ran-GAP1 (Ran-GTPase activating protein) by increasing GTP hydrolysis. RanBP1 also inhibits the function of the guanine nucleotide exchange factor RCC1 (regulator of chromosome condensation). Therefore, RanBP1 is responsible for the GTP-bound state of Ran and the increased GTP hydrolysis of Ran GTP.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 29 kDa

Comments:

Related Products: ABIN967389, ABIN968587

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of RanBP1 on HepG2 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of RanBP1.

Immunofluorescence staining of Human Fibroblast cells.

anti-RAN Binding Protein 1 (RANBP1) (AA 28-163) antibody (Image 3)

Publications

Product: Hayashi, Yokoyama, Seki et al.: "RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4." in: Molecular & general genetics : MGG, Vol. 247, Issue 6, pp. 661-9, 1995 (PubMed).

Bischoff, Krebber, Smirnova et al.: "Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1." in: The EMBO journal, Vol. 14, Issue 4, pp. 705-15, 1995 (PubMed).

Chi, Adam, Visser et al.: "RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import." in: The Journal of cell biology, Vol. 135, Issue 3, pp. 559-69, 1996 (PubMed).