RAN Binding Protein 1 (RANBP1) (AA 28-163) antibody
|Synonyms||HTF9A, MGC88701, Htf9a, MGC64314, ranbp2, MGC76028, RANBP1, MGC127635, MZN1.4, MZN1_4, RAN BINDING PROTEIN 1, zeh1094, zgc:85649, hm:zeh1094, wu:fa96a09, wu:fj39g07|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Description||Ran is a highly conserved GTPase that is ubiquitously expressed. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis, and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 29kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1. In addition, RanBP1 appears to regulate the activity of Ran-GAP1 (Ran-GTPase activating protein) by increasing GTP hydrolysis. RanBP1 also inhibits the function of the guanine nucleotide exchange factor RCC1 (regulator of chromosome condensation). Therefore, RanBP1 is responsible for the GTP-bound state of Ran and the increased GTP hydrolysis of Ran GTP.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||29 kDa|
Related Products: ABIN967389, ABIN968587
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Chromatin and Nuclear Signaling, DNA/RNA|
|Restrictions||For Research Use only|
|Western blot analysis of RanBP1 on HepG2 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of RanBP1. Immunofluorescence staining of Human Fibroblast cells. anti-RAN Binding Protein 1 (RANBP1) (AA 28-163) antibody (Image 3)|
Hayashi, Yokoyama, Seki et al.: "RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4." in: Molecular & general genetics : MGG, Vol. 247, Issue 6, pp. 661-9, 1995 (PubMed).
Bischoff, Krebber, Smirnova et al.: "Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1." in: The EMBO journal, Vol. 14, Issue 4, pp. 705-15, 1995 (PubMed).
Chi, Adam, Visser et al.: "RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import." in: The Journal of cell biology, Vol. 135, Issue 3, pp. 559-69, 1996 (PubMed).
|Hosts||Rabbit (28), Goat (6)|
|Reactivities||Human (32), Cow (Bovine) (20), Mouse (Murine) (19), Rat (Rattus) (19), Chicken (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18)|
|Applications||Western Blotting (WB) (18), Immunofluorescence (IF) (17), ELISA (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunocytochemistry (ICC) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (3), N-Term (2)|