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TCEB1 antibody (Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C)) (AA 1-112)

Details for Product anti-TCEB1 Antibody No. ABIN968161, Supplier: Log in to see
Antigen
  • 2610043E24Rik
  • 2610301I15Rik
  • AA407206
  • AI987979
  • AW049146
  • SIII
  • eloC
  • TCEB1
  • fb09f09
  • wu:fb09f09
  • zgc:111990
  • zgc:92635
Epitope
AA 1-112
17
16
2
2
2
1
1
1
Reactivity
Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
53
13
10
5
4
2
2
2
2
1
Host
Mouse
45
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This TCEB1 antibody is un-conjugated
5
5
5
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
39
24
21
2
2
1
1
1
Supplier
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Immunogen Human SIII p15 aa. 1-112
Clone 56-SIIIp15
Isotype IgG1
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SIII p15 (TCEB1 Antibody Abstract)
Background SIII p15 is one of three polypeptides that comprise the approximate 140kDa RNA polymerase II transcription factor SIII. The other two polypeptides have the molecular weights of 110kDa and 18kDa. All three polypeptides are required for transcription factor SIII function. Although the polypeptides were identified and purified from rat liver nuclear extracts, tissue expression of this transcription factor appears to be widespread. RNA polymerase II recognizes the SIII transcription factor and in turn initiates transcription from these correctly initiated transcripts. A yeast transcription factor designated YES may be the yeast homolog to SIII.
Synonyms: Elongin C
Molecular Weight 15 kDa
Comment

Related Products: ABIN968587, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-TCEB1 antibody (Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C)) (AA 1-112) (ABIN968161) Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane...
Immunofluorescence (IF) image for anti-TCEB1 antibody (Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C)) (AA 1-112) (ABIN968161) Immunofluorescent staining of Human Fibroblasts.
Product cited in: Bradsher, Jackson, Conaway et al.: "RNA polymerase II transcription factor SIII. I. Identification, purification, and properties." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25587-93, 1994 (PubMed).

Background publications Kuznetsova, Meller, Schnell et al.: "von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2706-11, 2003 (PubMed).