Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C) (TCEB1) (AA 1-112) antibody

Antigen: Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C) (TCEB1)

Synonyms: SIII, MGC72822

Binding Site: AA 1-112

Clonality: Monoclonal (56)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968162

Additional Information

Alternative name: SIII p15

Immunogen: Human SIII p15

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Frog

Format: Liquid

Isotype: IgG1

Clone: 56

Description: SIII p15 is one of three polypeptides that comprise the approximate 140kDa RNA polymerase II transcription factor SIII. The other two polypeptides have the molecular weights of 110kDa and 18kDa. All three polypeptides are required for transcription factor SIII function. Although the polypeptides were identified and purified from rat liver nuclear extracts, tissue expression of this transcription factor appears to be widespread. RNA polymerase II recognizes the SIII transcription factor and in turn initiates transcription from these correctly initiated transcripts. A yeast transcription factor designated YES may be the yeast homolog to SIII.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 15 kDa

Comments:

Related Products: ABIN968587, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Elongin C/SIII p15 antibody

Immunofluorescent staining of Human Fibroblasts.

anti-Transcription Elongation Factor B (SIII), Polypeptide 1 (15kDa, Elongin C) (TCEB1) (AA 1-112) antibody (Image 3)

Publications

Product: Bradsher, Jackson, Conaway et al.: "RNA polymerase II transcription factor SIII. I. Identification, purification, and properties." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25587-93, 1994 (PubMed).

Kuznetsova, Meller, Schnell et al.: "von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2706-11, 2003 (PubMed).