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ARF3 antibody (AA 1-181)

ARF3 Reactivity: Human, Mouse, Rat, Chicken, Dog, Frog WB, IHC, IF, IP Host: Mouse Monoclonal 41-ARF3 unconjugated
Catalog No. ABIN968175
  • Target See all ARF3 Antibodies
    ARF3 (ADP-Ribosylation Factor 3 (ARF3))
    Binding Specificity
    • 9
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-181
    Reactivity
    Human, Mouse, Rat, Chicken, Dog, Frog
    Host
    • 20
    • 1
    Mouse
    Clonality
    • 20
    Monoclonal
    Conjugate
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARF3 antibody is un-conjugated
    Application
    • 14
    • 10
    • 9
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity
    Rat (Rattus), Mouse (Murine), Chicken, Dog (Canine), Frog
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human ARF3 aa. 1-181
    Clone
    41-ARF3
    Isotype
    IgG1
    Top Product
    Discover our top product ARF3 Primary Antibody
  • Comment

    Related Products: ABIN968545, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Lee, Stevens, Hsu, Tsai, Lee, Moss, Vaughan: "Expression in human endothelial cells of ADP-ribosylation factors, 20-kDa guanine nucleotide-binding proteins involved in the initiation of vesicular transport." in: Journal of molecular and cellular cardiology, Vol. 28, Issue 9, pp. 1911-20, (1997) (PubMed).

    Morinaga, Tsai, Moss, Vaughan: "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12856-60, (1996) (PubMed).

    Cockcroft, Thomas, Fensome, Geny, Cunningham, Gout, Hiles, Totty, Truong, Hsuan: "Phospholipase D: a downstream effector of ARF in granulocytes." in: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 523-6, (1994) (PubMed).

    Moss, Vaughan: "ADP-ribosylation factors, 20,000 M(r) guanine nucleotide-binding protein activators of cholera toxin and components of intracellular vesicular transport systems." in: Cellular signalling, Vol. 5, Issue 4, pp. 367-79, (1993) (PubMed).

    Kahn, Radding: "Separation of the presynaptic and synaptic phases of homologous pairing promoted by recA protein." in: The Journal of biological chemistry, Vol. 259, Issue 12, pp. 7495-503, (1984) (PubMed).

  • Target
    ARF3 (ADP-Ribosylation Factor 3 (ARF3))
    Alternative Name
    ARF3 (ARF3 Products)
    Synonyms
    5430400P17Rik antibody, AI854770 antibody, arf3 antibody, MGC76217 antibody, MGC79549 antibody, ARF3 antibody, ADP-ribosylation factor 3 antibody, ARF-LIKE 1 antibody, ARL1 antibody, ATARL1 antibody, F27A10.8 antibody, ARF1 antibody, si:dz202l16.1 antibody, wu:fc66h11 antibody, zgc:92190 antibody, ADP ribosylation factor 3 antibody, ADP-ribosylation factor 3 antibody, ADP ribosylation factor 1 antibody, ADP ribosylation factor 3 S homeolog antibody, ADP-ribosylation factor 3b antibody, ADP-ribosylation factor 3a antibody, ARF3 antibody, Arf3 antibody, arf1 antibody, arf3.S antibody, arf3b antibody, arf3a antibody
    Background
    The ADP-ribosylation factors (ARFs) belong to the multigene family of small GTPases capable of activating cholera toxin. ARFs fall into three different classes: Class I is composed of ARF-1, ARF-2, and ARF-3, Class II consists of ARF-4 and ARF-5, and Class III includes ARF6. Unique to ARFs is their lack of intrinsic GTP hydrolysis activity, a high affinity for GDP in a Mg2+-dependent manner, and phospholipid requirement for nucleotide exchange. ARFs are involved in intravesicular acidification of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. In addition, GTP and ARF-3 are required for the activation of phospholipase D (PLD), an early cellular response triggered by the binding of receptors on the cell surface in response to numerous extracellular signals. Although predominantly cytosolic, ARF-3 can be translocated to cellular membranes upon cellular stimulation. The transition between the GDP-bound ARF-3 to the GTP-ARF-3 is facilitated by a high molecular weight guanine nucleotide-exchange factor sensitive to brefeldin. ARF-3 has been reported to be the most abundant in brain, kidney, and liver.
    Synonyms: ADP Ribosylation Factor-3
    Molecular Weight
    20 kDa
    Pathways
    Inositol Metabolic Process, Cellular Glucan Metabolic Process
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