Neurogenin 3 (NEUROG3) (AA 9-148) antibody
|Synonyms||ngn3, Atoh5, Math4B, bHLHa7, Ngn3, Relax, ngn2, atoh4, ngn3a, NEUROG3, atoh5, ngn-3, bhlha7, math4b, neurog3|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|4 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Alternative name||Neurogenin 3|
|Immunogen||Mouse Neurogenin 3|
|Cross-Reactivity||Rat (Rattus), Human|
|Description||Neurogenins are a family of atonal-related bHLH (basic helix-loop-helix) proteins that have been implicated in vertebrate neurogenesis. Members of this family include Neurogenin 1, 2, and 3. In a search for proteins that control early neuronal development, Neurogenin 1 was identified as a novel, Neuro-D-related bHLH protein that is expressed prior to Neuro-D in mouse and Xenopus. Identification of Neurogenins 2 and 3 defined the Neurogenin family. Analysis indicates that neurogenin gene expression defines progenitor populations in the developing nervous system and that neurogenin proteins and Neuro-D function in a cascade. Neurogenin proteins are expressed prior to Neuro-D and subsequent to another bHLH protein, MASH7. Neurogenin 3 is a 244 amino acid, 23 kDa protein that is expressed in fetal, neonatal, and neural tissues, as well as in several neuronal cell lines. Temporal and spatial expression of the neurogenin proteins is critical to proper neural determination and differentiation. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||23 kDa|
Related Products: ABIN968539, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of Neurogenin 3 on PC12 cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Neurogenin 3. anti-Neurogenin 3 (NEUROG3) (AA 9-148) antibody (Image 2)|
Ma, Kintner, Anderson: "Identification of neurogenin, a vertebrate neuronal determination gene." in: Cell, Vol. 87, Issue 1, pp. 43-52, 1996 (PubMed).
Sommer, Ma, Anderson: "neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS." in: Molecular and cellular neurosciences, Vol. 8, Issue 4, pp. 221-41, 1997 (PubMed).
Cau, Gradwohl, Fode et al.: "Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors." in: Development (Cambridge, England), Vol. 124, Issue 8, pp. 1611-21, 1997 (PubMed).
Ma, Sommer, Cserjesi et al.: "Mash1 and neurogenin1 expression patterns define complementary domains of neuroepithelium in the developing CNS and are correlated with regions expressing notch ligands." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 10, pp. 3644-52, 1997 (PubMed).
|Hosts||Rabbit (30), Mouse (7), Guinea Pig (1)|
|Reactivities||Human (33), Mouse (Murine) (24), Cow (Bovine) (18), Dog (Canine) (18), Rat (Rattus) (18), Sheep (Ovine) (18)|
|Applications||Western Blotting (WB) (21), Immunofluorescence (IF) (16), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (5), AA 1-50 (2), Center (1), Internal Region (1)|