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PRKAB1 antibody (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit) (AA 109-247)

Details for Product anti-PRKAB1 Antibody No. ABIN968185, Supplier: Login to see
Antigen
  • MGC82489
  • AMPK
  • HAMPKb
  • 1300015D22Rik
  • AU021155
  • E430008F22
Alternatives
anti-Human PRKAB1 antibody for Western Blotting
Epitope
AA 109-247
43
21
17
13
13
10
7
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
153
94
83
3
3
3
1
Host
Mouse
155
11
Clonality (Clone)
Monoclonal ()
Conjugate
This PRKAB1 antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
121
71
66
33
24
11
8
7
2
1
1
1
Supplier
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Immunogen Rat AMPK beta aa. 109-247
Clone 12-AMPKbeta
Isotype IgG1
Cross-Reactivity Mouse (Murine), Human, Dog (Canine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name AMPK-beta (PRKAB1 Antibody Abstract)
Background AMP activated protein kinase (AMPK) was identified as a result of its phosphorylation and inactivation of lipid metabolism enzymes. However, AMPK also phosphorylates enzymes involved in other metabolic pathways. AMPK is activated by AMPKK (AMPK Kinase)n-mediated phosphorylation in response to intracellular changes in AMP levels. Although rat skeletal muscle AMPK exists as a monomer, active rat liver AMPK is a heterotrimer of alpha (catalytic subunit), beta, and gamma subunits. The catalytic subunit (63 kDa) is structurally and functionally related to the S. cerevisiae SNF1 protein kinase, an enzyme that is required for expression of glucose repressed genes during glucose starvation. AMPK beta (38 kDa) is strongly related to SIP2 (35% identity) and contains a C-terminal region that is very homologous to the SIP1/SIP2/GAL83 ASC domain. AMPK beta has some identity (35%) with SNF4, which is necessary for SNF1 activation. Both AMPK beta and gamma are widely expressed in rat tissues. AMPK beta facilitates the interactions of the alpha and gamma subunits in vitro. Although AMPK beta is clearly necessary for formation of the heterotrimeric complex, there are other probably, yet unknown, roles for this protein.
Synonyms: AMP activated protein kinase
Molecular Weight 38 kDa
Pathways AMPK Signaling
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-PRKAB1 antibody (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit) (AA 109-247) (ABIN968185) Western blot analysis of AMPK beta on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1...
 image for anti-PRKAB1 antibody (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit) (AA 109-247) (ABIN968185) anti-Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 109-247) antibody (Image 2)
Western Blotting (WB) image for anti-PRKAB1 antibody (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit) (AA 109-247) (ABIN968185) anti-Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 109-247) antibody (Image 3)
Product cited in: Mitchelhill, Michell, House et al.: "Posttranslational modifications of the 5'-AMP-activated protein kinase beta1 subunit." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24475-9, 1997 (PubMed).

Winder, Wilson, Hardie et al.: "Phosphorylation of rat muscle acetyl-CoA carboxylase by AMP-activated protein kinase and protein kinase A." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 82, Issue 1, pp. 219-25, 1997 (PubMed).

Michell, Stapleton, Mitchelhill et al.: "Isoform-specific purification and substrate specificity of the 5'-AMP-activated protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 45, pp. 28445-50, 1996 (PubMed).

Woods, Cheung, Smith et al.: "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 17, pp. 10282-90, 1996 (PubMed).