Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 4-126) antibody

Antigen: Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7)

Synonyms: MCH3, CMH-1, ICE-LAP3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, mch3, cmh-1, ice-lap3, CASP7, LOC100101591

Binding Site: AA 4-126

Clonality: Monoclonal (51)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968191

Additional Information

Alternative name: Caspase-7

Immunogen: Human MCH-3

Cross-Reactivity: Dog (Canine)

Format: Liquid

Isotype: IgG2b

Clone: 51

Description: Apoptosis is induced by cysteine proteases which include Ced-3 of C.elegans, Caspase-3 (also known as CPP32, Yama, or apopain), and DCP-1 of Drosophila.. Multiple signals, such as activation of Fas and granzyme B, activate Caspase-3 which, in turn, cleaves substrates. Some substrates of Caspase-3 are PARP, MCH-3, MCH-2, U1-associated 70kDa protein, and SREBPs. Caspase-7/MCH-3 is a putative cysteine protease cloned from human Jurkat T lymphocytes. This protein shows significant homology to mammalian interleukin-1beta -converting enzyme (ICE), Caspase-3, and the Caenorhabditis elegans protein Ced3. All three of these proteins are cytoplasmic cysteine proteases that induce apoptosis when overexpressed in different cell types. This apoptosis can be inhibited by coexpression of Bcl-2. Similar to ICE and Caspase-3, Caspase-7 is a proenzyme that is proteolytically cleaved into p20 and p12 subunits that form the active Caspase-7 heterodimeric complex. Similarity to ICE and Caspase-3, as well as its high levels observed in lymphocytes, suggests that Caspase-7 plays an important role in immunologic apoptosis.
Synonyms: MCH-3

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 35 kDa

Comments:

Related Products: ABIN968587, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis Capase-7 on HepG2 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Capase-7.

anti-Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (AA 4-126) antibody (Image 2)

Publications

Product: Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).

Schlottman, Wachs, Krieg et al.: "Characterization of bile salt-induced apoptosis in colon cancer cell lines." in: Cancer research, Vol. 60, Issue 15, pp. 4270-6, 2000 (PubMed).

LeNegrate, Selva, Auberger et al.: "Sustained polymorphonuclear leukocyte transmigration induces apoptosis in T84 intestinal epithelial cells." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1479-88, 2000 (PubMed).

Li, Marani, Yu et al.: "Adenovirus-mediated Bax overexpression for the induction of therapeutic apoptosis in prostate cancer." in: Cancer research, Vol. 61, Issue 1, pp. 186-91, 2001 (PubMed).

Deschesnes, Huot, Valerie et al.: "Involvement of p38 in apoptosis-associated membrane blebbing and nuclear condensation." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1569-82, 2001 (PubMed).