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Flotillin 1 antibody (FLOT1) (AA 312-428)

Details for Product anti-FLOT1 Antibody No. ABIN968195, Supplier: Login to see New
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Synonyms flot1, flot1c, flotillin-1, flotillin1, flot1a, reggie-2, Rareg, reggie2, si:dz44o19.1, wu:fj45g11
AA 312-428
(16), (8), (8), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chicken, Human, Mouse (Murine), Rat (Rattus)
(59), (14), (13), (4), (4), (4), (2), (1), (1)
(41), (16), (4)
Clonality (Clone)
Monoclonal ()
This Flotillin 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Immunofluorescence (IF), Western Blotting (WB)
(56), (27), (6), (5), (5), (4), (2)
Pubmed 5 references available
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Quantity 150 μg
Shipping to United States ( )
Immunogen Mouse Flotillin aa. 312-428
Clone 18-Flotillin
Isotype IgG1
Cross-Reactivity Rat (Rattus), Chicken, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Flotillin-1 (FLOT1 Antibody Abstract)
Background Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCalpha, Ras, Rap Src-like kinases, Galphaßgamma,and GPI-linked receptors. This antibody is routinely tested by western blot analysis.
Molecular Weight 48 kDa

Related Products: ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Flotillin 1 antibody (FLOT1) (AA 312-428) (ABIN968195) Western blot analysis of flotillin-1 on rat brain lysate. Lane 1: 1:250, Lane 2: 1:50...
 image for anti-Flotillin 1 antibody (FLOT1) (AA 312-428) (ABIN968195) anti-Flotillin 1 (FLOT1) (AA 312-428) antibody (Image 2)
Product cited in: Cabin, Shimazu, Murphy et al.: "Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 20, pp. 8797-807, 2002 (PubMed).

Morrow, Rea, Martin et al.: "Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48834-41, 2002 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Rybin, Xu, Lisanti et al.: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, 2001 (PubMed).

Bickel, Scherer, Schnitzer et al.: "Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13793-802, 1997 (PubMed).

Catalog No. ABIN968195
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