ITGAL antibody (AA 64-199)

Catalog No. ABIN968200

Product Details anti-ITGAL Antibody

Target: ITGAL (Integrin alpha-L (ITGAL))

Binding Specificity: AA 64-199

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LFA-1alpha aa. 64-199

Clone: 27-CD11a

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ITGAL antibody (ABIN968200)

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, (2002) (PubMed).

Lub, van Kooyk, van Vliet, Figdor: "Dual role of the actin cytoskeleton in regulating cell adhesion mediated by the integrin lymphocyte function-associated molecule-1." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 341-51, (1997) (PubMed).

Nueda, López-Cabrera, Vara, Corbí: "Characterization of the CD11a (alpha L, LFA-1 alpha) integrin gene promoter." in: The Journal of biological chemistry, Vol. 268, Issue 26, pp. 19305-11, (1993) (PubMed).

Larson, Corbi, Berman, Springer: "Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily." in: The Journal of cell biology, Vol. 108, Issue 2, pp. 703-12, (1989) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a (ITGAL Products)

Synonyms: CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody

Background: CD11a, also known as integrin alphaL or LFA-1 (lymphocyte function-associated antigen-1), is a member of the beta2 family of integrins that includes Mac-1 and p150,95. It is composed of two noncovalently linked alpha and beta chains which are associated with the actin cytoskeleton. CD11a (integrin alphaL) has been reported to be expressed on the surface of T cells, neutrophils, and macrophages. It is involved in cell-cell adhesion and it also conveys signals from the plasma membrane to the cytoplasm. Activation of the TCR/CD3 complex on T cells transmits intracellular signals that activate integrin alphaL (inside-out” signaling). This results in the conformational change of integrin alphaL, which increases its affinity for its ligand ICAM-1,-2, and -3. Furthermore, this signaling releases integrin alphaL from the actin cytoskeleton, resulting in clustering of this surface molecule. The high affinity conformation and the clustering of integrin alphaL increases the adhesion between it and ICAM. This adhesion is essential for leukocyte activation and results in outside-in” signaling which induces the polymerization of actin, thereby stabilizing the integrin alphaL-ICAM interaction. This interaction is necessary for CTL and NK mediated killing, T and B cell responses, ADCC, and immune cell extravasation. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alphaL, LFA-1alpha

Molecular Weight: 180 kDa

Pathways: Activated T Cell Proliferation, Integrin Complex