Stress-Induced-phosphoprotein 1 (STIP1) (AA 22-143) antibody

Antigen: Stress-Induced-phosphoprotein 1 (STIP1)

Synonyms: HOP, P60, STI1, STI1L, IEF-SSP-3521, Hop, p60, Sti1, MGC53256, STIP1, stip1, MGC76181, MGC82554, zgc:92133, MGC128110

Binding Site: AA 22-143

Clonality: Monoclonal (28)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968204

Additional Information

Alternative name: STI1

Immunogen: Human STI1

Cross-Reactivity: Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 28

Description: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. The human Stress-Inducible protein 1 (STI1) was identified from SV40-transformed MRC-5 fibroblasts and shares 42% homology with stress-inducible mediator for the heat shock response in yeast. STI1, which contains the tetratricopeptide (TPR) motif widely represented in proteins required for mitosis and RNA synthesis, is induced in SV40-transformed cells and by heat shock. STI1 was also detected in macromolecular complexes with the heat shock proteins HSP70, 84/86, and 90. Further studies showed that STI1 helps in the maturation of several protein targets of HSP90. Immunolocalization analysis localized STI1 within the nucleus of transformed cells, whereas the same protein was found in the Golgi region of normal cells.
Synonyms: Stress-Inducible protein 1, Hop-p60

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 62 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of STI1 on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-STI1 antibody.

Immunofluorescence staining of FHs cells (Normal human fetal lung fibroblasts, ATCC HTB-157).

anti-Stress-Induced-phosphoprotein 1 (STIP1) (AA 22-143) antibody (Image 3)

Publications

Product: Honoré, Leffers, Madsen et al.: "Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1." in: The Journal of biological chemistry, Vol. 267, Issue 12, pp. 8485-91, 1992 (PubMed).

Chang, Nathan, Lindquist: "In vivo analysis of the Hsp90 cochaperone Sti1 (p60)." in: Molecular and cellular biology, Vol. 17, Issue 1, pp. 318-25, 1997 (PubMed).

Lässle, Blatch, Kundra et al.: "Stress-inducible, murine protein mSTI1. Characterization of binding domains for heat shock proteins and in vitro phosphorylation by different kinases." in: The Journal of biological chemistry, Vol. 272, Issue 3, pp. 1876-84, 1997 (PubMed).