Did you know that you can buy products from over 140 different suppliers from us?

Smad2/3 Transcription Factor (SMAD2/3) (AA 142-263) antibody

Details for Product No. ABIN968208
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
AA 142-263
(9), (5), (3), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(30), (19), (17), (1)
Host
Mouse
(28), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(27), (9), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 7 references available
Quantity 50 µg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968208
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Mouse Smad2
Clone 18
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Smad2/3
Background The transforming growth factor beta (TGFbeta)/activin/BMP family of growth factors plays a diverse and important role in growth, development, and differentiation. These growth factors act through their binding to heteromeric plasma membrane receptor protein kinases which, upon ligand binding, become activated and trigger an intracellular signaling cascade. Specifically, receptor activation induces the translocation of a set of conserved proteins named Smads (Sma- and Mad-related proteins) to the nucleus, resulting in gene activation. Smad2 is a ubiquitously expressed protein of 58 kDa that is phosphorylated and translocated to the nucleus in response to TGFbeta, but not BMP. The overall response to TGFbeta is growth inhibition. The Smad2 gene is located in chromosome 18q21.1 which is often absent in several human cancers. Furthermore, some missense mutations on the Smad2 gene were identified in colorectal carcinomas, suggesting Smad2 may function as a tumor suppressor in normal cells. Investigators should note that potential crossreactivity to Smad3 is predicted based on sequence homology of the immunogen, Mouse Smad2 aa. 142-263. In addition, reactivity to mouse Smad2, using siRNA knockdown, has recently been described (Dzwonek et al.). Reactivity to canine Smad3 has also been reported using nuclear extracts (Lehman et al.).
Molecular Weight 58 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Smad2/3 Transcription Factor (SMAD2/3) (AA 142-263) antibody Western blot analysis of Smad2/3 on Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Smad2/3.
anti-Smad2/3 Transcription Factor (SMAD2/3) (AA 142-263) antibody (2) HISM cells grown on microscope slides and stained with Smad2/3 antibody.
Product cited in: Lechleider, de Caestecker, Dehejia et al.: "Serine phosphorylation, chromosomal localization, and transforming growth factor-beta signal transduction by human bsp-1." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17617-20, 1996 (PubMed).

Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).

Lehmann, Janda, Pierreux et al.: "Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells." in: Genes & development, Vol. 14, Issue 20, pp. 2610-22, 2000 (PubMed).

Hocevar, Smine, Xu et al.: "The adaptor molecule Disabled-2 links the transforming growth factor beta receptors to the Smad pathway." in: The EMBO journal, Vol. 20, Issue 11, pp. 2789-801, 2001 (PubMed).

Hayes, Chawla, Corvera: "TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1239-49, 2002 (PubMed).

Luo, Nieves, Kzhyshkowska et al.: "Endogenous transforming growth factor-beta receptor-mediated Smad signaling complexes analyzed by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 7, pp. 1245-60, 2006 (PubMed).

Dzwonek, Preobrazhenska, Cazzola et al.: "Smad3 is a key nonredundant mediator of transforming growth factor beta signaling in Nme mouse mammary epithelial cells." in: Molecular cancer research : MCR, Vol. 7, Issue 8, pp. 1342-53, 2009 (PubMed).

Validation Images
back to top