Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 15-133) antibody

Details for Product No. ABIN968216
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Antigen
Synonyms UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, C79827, Ubce7, ube2l3l, wu:fb38h03
Epitope
AA 15-133
(24), (10), (9), (7), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(71), (29), (27), (17), (4), (3), (3), (3), (3), (1)
Host
Mouse
(55), (9), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(61), (32), (16), (14), (10), (10), (9), (3)
Pubmed 3 references available
Quantity 150 μg
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Catalog No. ABIN968216
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Immunogen Human UbcH7
Clone LEP-02
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name UbcH7
Background Progression of the mammalian cell cycle is regulated mainly by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome-a cytoplasmic complex of proteases. Several human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been identified and found involved in critical processes such as cell cycle progression and apoptosis. UbcH7 was characterized as an E6-AP-interacting protein required for the ubiquitination of p53. E6-AP is the ubiquitin ligase used as bait in the search for interacting proteins required for the proper ubiquitination of cellular targets. UbcH7 interacts with the C-terminal region of E6-AP named the hect domain. Therefore, UbcH7 is another protein, like Ubc9, involved in the degradation and regulation of critical proteins in the cell cycle. This antibody is routinely tested by western blot analysis.
Synonyms: Ubiquitin Conjugating Enzyme H7
Molecular Weight 17-18 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 15-133) antibody Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 15-133) antibody (2) Western blot analysis of UbcH7 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-UbcH7 antibody.
anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 15-133) antibody (3) anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 15-133) antibody (Image 3)
Product cited in: Nuber, Schwarz, Kaiser et al.: "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2795-800, 1996 (PubMed).

Shimura, Schlossmacher, Hattori et al.: "Ubiquitination of a new form of alpha-synuclein by parkin from human brain: implications for Parkinson's disease." in: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 263-9, 2001 (PubMed).

Hagglund, Van Sant, Lopez et al.: "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 631-6, 2002 (PubMed).

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