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SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 359-473)

Details for Product anti-SHC1 Antibody No. ABIN968228, Supplier: Login to see
Antigen
  • SHC1
  • SHC
  • SHCA
  • P66shc
  • si:dkey-246i14.4
  • wu:fc10h03
  • shc
  • p66shc
  • Shc
  • ShcA
  • p66
Alternatives
anti-Human SHC1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 359-473
44
41
17
15
13
13
13
11
9
9
9
8
7
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
232
170
131
9
6
5
2
2
1
1
1
Host
Mouse
215
28
Clonality (Clone)
Monoclonal ()
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
181
83
67
56
49
38
14
9
7
6
4
1
1
Supplier
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Immunogen Human SHC aa. 359-473
Clone 30-SHC
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SHC (SHC1 Antibody Abstract)
Background The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.
Molecular Weight 66, 52 & 46 kDa
Research Area Signaling, Alzheimer's Disease, Tyrosine Kinases
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 359-473) (ABIN968228) Western blot analysis of SHC on a HeLa cell lysate (Human cervical epitheloid carcino...
Immunofluorescence (IF) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 359-473) (ABIN968228) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).
Product cited in: Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, 2002 (PubMed).

Ugi, Imamura, Ricketts et al.: "Protein phosphatase 2A forms a molecular complex with Shc and regulates Shc tyrosine phosphorylation and downstream mitogenic signaling." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2375-87, 2002 (PubMed).

Laser, Willey, Jiang et al.: "Integrin activation and focal complex formation in cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35624-30, 2000 (PubMed).

Imokawa, Kobayasi, Miyagishi: "Intracellular signaling mechanisms leading to synergistic effects of endothelin-1 and stem cell factor on proliferation of cultured human melanocytes. Cross-talk via trans-activation of the tyrosine kinase c-kit receptor." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33321-8, 2000 (PubMed).

Pelicci, Lanfrancone, Grignani et al.: "A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction." in: Cell, Vol. 70, Issue 1, pp. 93-104, 1992 (PubMed).