SHC1 antibody (AA 359-473)

Catalog No. ABIN968228

Product Details anti-SHC1 Antibody

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Binding Specificity: AA 359-473

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SHC aa. 359-473

Clone: 30-SHC

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SHC1 antibody (ABIN968228)

Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, (2002) (PubMed).

Ugi, Imamura, Ricketts, Olefsky: "Protein phosphatase 2A forms a molecular complex with Shc and regulates Shc tyrosine phosphorylation and downstream mitogenic signaling." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2375-87, (2002) (PubMed).

Imokawa, Kobayasi, Miyagishi: "Intracellular signaling mechanisms leading to synergistic effects of endothelin-1 and stem cell factor on proliferation of cultured human melanocytes. Cross-talk via trans-activation of the tyrosine kinase c-kit receptor." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33321-8, (2000) (PubMed).

Laser, Willey, Jiang, Cooper, Menick, Zile, Kuppuswamy: "Integrin activation and focal complex formation in cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35624-30, (2000) (PubMed).

Pelicci, Lanfrancone, Grignani, McGlade, Cavallo, Forni, Nicoletti, Grignani, Pawson, Pelicci: "A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction." in: Cell, Vol. 70, Issue 1, pp. 93-104, (1992) (PubMed).

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: SHC (SHC1 Products)

Synonyms: SHC1 antibody, SHC antibody, SHCA antibody, P66shc antibody, si:dkey-246i14.4 antibody, wu:fc10h03 antibody, shc antibody, p66shc antibody, Shc antibody, ShcA antibody, p66 antibody, SHC adaptor protein 1 antibody, Shc1p antibody, squalene--hopene cyclase antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antibody, src homology 2 domain-containing transforming protein C1 antibody, SHC1 antibody, shc1 antibody, Shc1 antibody, shc1.L antibody

Background: The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.

Molecular Weight: 66, 52 & 46 kDa

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2