Rab4 antibody (AA 97-213)

Catalog No. ABIN968232

Product Details anti-Rab4 Antibody

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Binding Specificity: AA 97-213

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rab4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rab4 aa. 97-213

Clone: 7-Rab4

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Rab4 antibody (ABIN968232)

Steiner, Sarria, Glauser, Magnin, Catsicas, Hirling: "Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1197-209, (2002) (PubMed).

Sans, Racca, Petralia, Wang, McCallum, Wenthold: "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 19, pp. 7506-16, (2001) (PubMed).

Cormont, Bortoluzzi, Gautier, Mari, van Obberghen, Le Marchand-Brustel: "Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6879-86, (1997) (PubMed).

Zahraoui, Touchot, Chardin, Tavitian: "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion." in: The Journal of biological chemistry, Vol. 264, Issue 21, pp. 12394-401, (1989) (PubMed).

Target Details for Rab4

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Alternative Name: Rab4 (RAB4A Products)

Synonyms: rab4a antibody, MGC52945 antibody, fc93b03 antibody, wu:fc93b03 antibody, RAB4A antibody, HRES-1/RAB4 antibody, RAB4 antibody, AI848268 antibody, Rab4 antibody, RAB4A, member RAS oncogene family antibody, RAB4a, member RAS oncogene family antibody, rab4a antibody, RAB4A antibody, Rab4a antibody

Background: The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking. The Rab proteins cycle between active GTP-bound and inactive GDP-bound states. Rab4 is associated with early endosomes and recycling vesicles. It is involved in the recycling of receptors to the plasma membrane and regulates membrane recycling from early endosomes. Rab4 has been reported to be membrane-bound during interphase. However, it can be found as a cytosolic protein during mitosis. It is thought that Rab4 is phosphorylated by cdc2/cyclin B during mitosis and that Ser196 on Rab4 is the recognition site of cdc2. Mutation of Ser196 results in Rab4 membrane association during mitosis in an unphosphorylated form. Together this data indicates that Rab4 is phosphorylated by cdc2 on Ser196 which releases it into the cytosol during mitosis. In addition, Rab4 may play a role in insulin-induced glucose transport into rat adipocytes. Rab4 has also been reported to be associated with vesicles that contain GLUT4, a glucose transporter isoform. This antibody is routinely tested by western blot analysis.

Molecular Weight: 25 kDa