RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody

Details for Product No. ABIN968233
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Antigen
Synonyms rab4a, MGC52945, fc93b03, wu:fc93b03, RAB4A, HRES-1/RAB4, RAB4, AI848268, Rab4
Epitope
AA 97-213
(7), (6), (4), (1), (1), (1), (1)
Reactivity
Human
(46), (21), (20), (12), (12), (12), (12), (12), (4)
Host
Mouse
(27), (18), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(35), (18), (10), (7), (7), (5), (4), (3), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 150 μg
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Catalog No. ABIN968233
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Immunogen Human Rab4
Clone 7
Isotype IgG1
Cross-Reactivity Rat (Rattus), Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Rab4
Background The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking. The Rab proteins cycle between active GTP-bound and inactive GDP-bound states. Rab4 is associated with early endosomes and recycling vesicles. It is involved in the recycling of receptors to the plasma membrane and regulates membrane recycling from early endosomes. Rab4 has been reported to be membrane-bound during interphase. However, it can be found as a cytosolic protein during mitosis. It is thought that Rab4 is phosphorylated by cdc2/cyclin B during mitosis and that Ser196 on Rab4 is the recognition site of cdc2. Mutation of Ser196 results in Rab4 membrane association during mitosis in an unphosphorylated form. Together this data indicates that Rab4 is phosphorylated by cdc2 on Ser196 which releases it into the cytosol during mitosis. In addition, Rab4 may play a role in insulin-induced glucose transport into rat adipocytes. Rab4 has also been reported to be associated with vesicles that contain GLUT4, a glucose transporter isoform. This antibody is routinely tested by western blot analysis.
Molecular Weight 25 kDa
Research Area Cancer, Organelles, Signaling
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody Immunofluorescence staining of L6 cells (rat skeletal muscle myoblasts, ATCC CRL-1458).
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody (2) Western blot analysis of Rab4 on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Rab4 antibody.
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody (3) anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 97-213) antibody (Image 3)
Product cited in: Zahraoui, Touchot, Chardin et al.: "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion." in: The Journal of biological chemistry, Vol. 264, Issue 21, pp. 12394-401, 1989 (PubMed).

Cormont, Bortoluzzi, Gautier et al.: "Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6879-86, 1997 (PubMed).

Sans, Racca, Petralia et al.: "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 19, pp. 7506-16, 2001 (PubMed).

Huang, Imamura, Olefsky: "Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13084-9, 2001 (PubMed).

Steiner, Sarria, Glauser et al.: "Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1197-209, 2002 (PubMed).

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