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Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody

Details for Product No. ABIN968234, Supplier: Log in to see
Antigen
  • AP3B2
  • DDBDRAFT_0217414
  • DDBDRAFT_0238781
  • DDB_0217414
  • DDB_0238781
  • NAPTB
  • AI549966
  • AU042881
  • Naptb
  • [b]-NAP
  • beta-NAP
  • beta3B
Epitope
AA 839-1036
26
15
2
2
1
Reactivity
Mouse (Murine), Rat (Rattus)
35
8
8
Host
Mouse
33
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
33
22
4
3
2
1
Supplier
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Immunogen Human beta-NAP aa. 839-1036
Clone 18-beta
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name beta-NAP (AP3B2 Antibody Abstract)
Background Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. Two AP-3 complexes have been identified as a neuronal complex and a ubiquitously-expressed complex. The neuronal AP-3 complex contains Adaptin beta3B (beta-NAP), which has 61% identify with Adaptin beta3A found in the ubiquitously-expressed AP-3 complex, and 30% identity with Adaptins beta1 and beta2 found in the AP-1 and AP-2 complexes.
Molecular Weight 145 kDa
Pathways
Comment

Related Products: ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody (ABIN968234) Western blot analysis of beta-NAP on rat brain lysate. Lane 1: 1:500, lane 2: 1: 1000...
 image for anti-Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody (ABIN968234) anti-Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody (Image 2)
Western Blotting (WB) image for anti-Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody (ABIN968234) anti-Adaptor-Related Protein Complex 3, beta 2 Subunit (AP3B2) (AA 839-1036) antibody (Image 3)
Product cited in: DellAngelica, Ooi, Bonifacino: "Beta3A-adaptin, a subunit of the adaptor-like complex AP-3." in: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15078-84, 1997 (PubMed).

Simpson, Peden, Christopoulou et al.: "Characterization of the adaptor-related protein complex, AP-3." in: The Journal of cell biology, Vol. 137, Issue 4, pp. 835-45, 1997 (PubMed).

Newman, McKeever, Okano et al.: "Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein." in: Cell, Vol. 82, Issue 5, pp. 773-83, 1995 (PubMed).