TGOLN2 antibody (AA 31-244)

Catalog No. ABIN968238

Product Details anti-TGOLN2 Antibody

Target: TGOLN2 (Trans-Golgi Network Protein 2 (TGOLN2))

Binding Specificity: AA 31-244

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGOLN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat TGN38 aa. 31-244

Clone: 2-TGN38

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TGOLN2 antibody (ABIN968238)

Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

Ozawa, Kondo, Hori, Kitao, Stern, Eisenmenger, Ogawa, Ohshima: "Expression of the oxygen-regulated protein ORP150 accelerates wound healing by modulating intracellular VEGF transport." in: The Journal of clinical investigation, Vol. 108, Issue 1, pp. 41-50, (2001) (PubMed).

Xu, Shen, Joseph, Bryant, Luo, Frankel, Rotunda, Foster: "Mitogenic phospholipase D activity is restricted to caveolin-enriched membrane microdomains." in: Biochemical and biophysical research communications, Vol. 273, Issue 1, pp. 77-83, (2000) (PubMed).

Humphrey, Peters, Yuan, Bonifacino: "Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence." in: The Journal of cell biology, Vol. 120, Issue 5, pp. 1123-35, (1993) (PubMed).

Target Details for TGOLN2

Target: TGOLN2 (Trans-Golgi Network Protein 2 (TGOLN2))

Alternative Name: TGN38 (TGOLN2 Products)

Synonyms: TGN38B antibody, TGN41 antibody, TGN46 antibody, Ttgn2 antibody, TGN38 antibody, TGN48 antibody, TGN51 antibody, TTGN2 antibody, TGOLN2 antibody, DKFZp469D101 antibody, Tgn38 antibody, Tgoln1 antibody, Ttgn1 antibody, trans-golgi network protein 2 antibody, Tgoln2 antibody, TGOLN2 antibody

Background: Newly synthesized proteins exit the ER and move to the cis-Golgi network (CGN) where they traverse the cis-medial and trans-cisternae before reaching the trans-Golgi network (TGN). N-linked oligosaccharide processing occurs in the TGN, and proteins are sorted to the plasma membrane, lysosomes, endosomes, and secretory granules. TGN38 is a type I integral membrane protein primarily localized to the TGN. It is involved in the sorting of nascent proteins into individual carrier vesicles for transport to appropriate destinations. It is thought to heterodimerize with TGN41 and participate in exocytic budding from the TGN. TGN38 has a molecular weight of 85 to 95 kDa. The core polypeptide represents approximately 38 kDa, while the remainder is accounted for by N- and O-linked oligosaccharide chains. A 286 aa N-terminal lumenal domain, a 21 aa membrane spanning domain, and a 33 aa C-terminal cytoplasmic tail comprise the structure of TGN38. The cytoplasmic tail contains a tyrosine-based motif, YQRL, that is thought to be involved in TGN localization. Therefore, TGN38 mediates the localization of various proteins to the TGN and serves as a TGN retrieval signal. This antibody is routinely tested by western blot analysis.
Synonyms: Trans Golgi Network-38

Molecular Weight: 85-95 kDa