phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (AA 4-159) antibody

Details for Product No. ABIN968253
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Antigen
Synonyms PLCB1, 3110043I21Rik, AI132408, Plcb, mKIAA0581, EIEE12, PI-PLC, PLC-154, PLC-I, PLC154, PLCB1A, PLCB1B, PLC'1, PLCbeta1, Phosphb, RATPHOSPHB
Epitope
AA 4-159
(13), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(41), (25), (21), (14), (12), (12)
Host
Mouse
(40), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(26), (11), (10), (6), (4), (2), (2), (1)
Pubmed 4 references available
Quantity 50 μg
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Catalog No. ABIN968253
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Immunogen Rat Phospholipase Cbeta1
Clone Ghr6
Isotype IgG1
Cross-Reactivity Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Phospholipase C beta 1
Background Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). The former causes release of Ca2+ from intracellular stores, while the latter is an activator of PKC. At least three isozymes are known: PLCbeta, PLCgamma, and PLCdelta. They recognize phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP2), and phospatidylinositol 4,5 bisphosphate (PIP2) and carry out the Ca2+-dependent hydrolysis of these inositol phospholipids. All three classes contain two highly conserved regions, designated X” and Y”, whose structural integrity is essential for a functional catalytic core. The PLCbeta subfamily has been reported to contain at least four members: beta1, beta2, beta3 and beta4. PLCbeta1 has been reported to have been identified and cloned from brain tissue where the PLCbeta1 gene encodes for two different mRNAs. The resulting protein products, PLCbeta1a (121 aa) and PLCbeta1b (1173 aa) differ only at their C-termini. Although PLCgamma activity is induced by receptor tyrosine kinase phosphorylation, PLCbeta is regulated by G protein alpha subunits or betagamma subunits. In particular, Galphaq has been reported to activate PLCbeta1. This antibody is routinely tested by western blot analysis.
Synonyms: PLCbeta1
Molecular Weight 150 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (AA 4-159) antibody Immunofluorescence staining of human intestinal smooth muscle cells (HISM).
anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (AA 4-159) antibody (2) Western blot analysis of Phospholipase Cbeta1 on a rat cerebrum lysate. Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti-phospholipase Cbeta1 antibody.
anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (AA 4-159) antibody (3) anti-phospholipase C, beta 1 (phosphoinositide-Specific) (PLCB1) (AA 4-159) antibody (Image 3)
Product cited in: Rhee, Choi: "Regulation of inositol phospholipid-specific phospholipase C isozymes." in: The Journal of biological chemistry, Vol. 267, Issue 18, pp. 12393-6, 1992 (PubMed).

Suh, Ryu, Moon et al.: "Cloning and sequence of multiple forms of phospholipase C." in: Cell, Vol. 54, Issue 2, pp. 161-9, 1988 (PubMed).

Bahk, Lee, Lee et al.: "Two forms of phospholipase C-beta 1 generated by alternative splicing." in: The Journal of biological chemistry, Vol. 269, Issue 11, pp. 8240-5, 1994 (PubMed).

Avazeri, Courtot, Pesty et al.: "Cytoplasmic and nuclear phospholipase C-beta 1 relocation: role in resumption of meiosis in the mouse oocyte." in: Molecular biology of the cell, Vol. 11, Issue 12, pp. 4369-80, 2000 (PubMed).

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