GTF2I antibody (AA 17-123)

Catalog No. ABIN968264

Product Details anti-GTF2I Antibody

Target: GTF2I (General Transcription Factor III (GTF2I))

Binding Specificity: AA 17-123

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GTF2I antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human BAP-135 aa. 17-123

Clone: 42-TFII

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-GTF2I antibody (ABIN968264)

Yang, Desiderio: "BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 2, pp. 604-9, (1997) (PubMed).

Target Details for GTF2I

Target: GTF2I (General Transcription Factor III (GTF2I))

Alternative Name: TFII-I (GTF2I Products)

Synonyms: BAP135 antibody, BTKAP1 antibody, DIWS antibody, GTFII-I antibody, IB291 antibody, SPIN antibody, TFII-I antibody, WBS antibody, WBSCR6 antibody, Gtf2ird1 antibody, 6030441I21Rik antibody, BAP-135 antibody, GtfII-I antibody, Spin antibody, bap-135 antibody, bap135 antibody, btkap1 antibody, diws antibody, gtfii-i antibody, ib291 antibody, spin antibody, tfii-i antibody, wbs antibody, wbscr6 antibody, gtf2i antibody, gtf2i.L antibody, general transcription factor IIi antibody, general transcription factor II I antibody, general transcription factor IIi S homeolog antibody, GTF2I antibody, Gtf2i antibody, gtf2i antibody, gtf2i.S antibody

Background: General transcription factor II-I (GTF2I/TFII-I/SPIN/BAP-135) is a transcription factor that contains six directly repeated 90-residue regions, which possess helix-loop-helix protein-protein interaction motifs. TFII-I can regulate transcription in T-cells through interaction with the initiator elments (Inrs) within the AdML and Vbeta promoters, and associates with HIV-1, TdT, and ribonucleotide reductase R1 Inrs. In addition, TFII-I associates with the E box motif, the CACGTG sequence, and with serum response element sequences. The helix-loop-helix repeats facilitate TFII-I interaction with other transcription factors, such as USF, Myc, Phox 1, MADS box protein serum response factor, and STATs. TFII-I was also identified as Bruton's tyrosine kinase (btk)-associated protein (BAP-135). TFII-I/BAP-135 is tyrosine phosphorylated by btk and after EGF stimulation, and this phosphorylation enhances TFII-I transcriptional activity. The wide expression of TFII-I and the interaction of TFII-I with various Inrs and transcription factors implicates TFII-I in various signaling pathways that regulate gene transcription.
Synonyms: BAP-135

Molecular Weight: 135/140 kDa