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Details for Product No. ABIN968266

FYN-Binding Protein (FYB) (AA 673-783) antibody

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Antigen
Synonyms ADAP, PRO0823, SLAP-130, B630013F22Rik, FYB-120/130, Slap130, p120/130, p120/p130, fyb
Epitope
»Alternatives AA 673-783
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF)
Pubmed 4 references available
Catalog no. ABIN968266
Quantity 150 µg
Price
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Immunogen Human FYB
Clone 5
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name FYB
Background Engagement of the T cell receptor (TcR) results in the activation of multiple intracellular src protein tyrosine kinases (PTKs) including p56 [lck] and p59 [fyn]. PTK activation induces TcRzeta phosphorylation and the subsequent recruitment and activation of ZAP-70 which specifically phosphorylates SLP-76. SLP-76 interacts with multiple components of T cell signaling pathways, including Grb-2, PLC-gamma, Vav, and SLAP-130 (also known as SLP-76 Associated Phosphoprotein of 130 kDa). SLAP-130 contains multiple tyrosine based motifs, proline base type I and II SH3 domain binding motifs, lysine/glutamic acid rich nuclear localization motifs, and an SH3-like domain. It associates with the SH2 domain of SLP-76 and is phosphorylated by TcR induced PTKs. This protein has also been reported to directly interact with fyn and has been referred to as fyn binding protein (FYB). Co-transfection data has shown that a limited concentration of FYB/SLAP-130 enhances TcR-induced IL-2 production, while overexpression downregulates gene expression. Thus, FYB/SLAP-130 is viewed to be a regulatory component of the fyn/SLP-76 signaling cascade in T cells.
Synonyms: Fyn Binding Protein, SLAP-130
Molecular Weight 130 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Musci, Hendricks-Taylor, Motto et al.: "Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases." in: The Journal of biological chemistry, Vol. 272, Issue 18, pp. 11674-7, 1997 (PubMed).

da Silva, Li, de Vera et al.: "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 14, pp. 7493-8, 1997 (PubMed).

Oda, Ikeda, Ochs et al.: "Rapid tyrosine phosphorylation and activation of Bruton's tyrosine/Tec kinases in platelets induced by collagen binding or CD32 cross-linking." in: Blood, Vol. 95, Issue 5, pp. 1663-70, 2000 (PubMed).

Geng, Pfister, Kraeft et al.: "Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11527-32, 2001 (PubMed).

Alternatives for antigen "FYN-Binding Protein (FYB)", type "Antibodies"
Hosts (47), (4), (3)
Reactivities (53), (20), (13), (13), (13), (12), (12), (1), (1), (1), (1)
Applications (44), (27), (20), (10), (5), (3), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (11), (2), (2), (2), (1), (1), (1)
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