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MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188)

Details for Product anti-MAP2K5 Antibody No. ABIN968273, Supplier: Log in to see
Antigen
  • Mek5
  • HsT17454
  • MAPKK5
  • MEK5
  • PRKMK5
  • AI324775
  • AI428457
  • Mapkk5
  • Prkmk5
  • fb73f02
  • wu:fb73f02
  • zgc:172137
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAP2K_A
Alternatives
anti-Dog (Canine) MAP2K5 antibody for Western Blotting
Epitope
AA 13-188
30
29
17
17
15
13
13
13
13
6
5
5
4
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
148
72
72
8
7
7
6
5
3
2
2
1
1
1
1
Host
Mouse
149
15
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
5
5
5
5
5
5
5
5
4
4
4
4
Application
Immunofluorescence (IF), Western Blotting (WB)
104
54
47
24
19
18
4
2
1
1
1
Supplier
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Immunogen Human MEK5 aa. 13-188
Clone 21-MEK5
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEK5 (MAP2K5 Antibody Abstract)
Background The mitogen-activated protein (MAP) kinase cascade participates in the Ras signal transduction pathway. The MAP kinase cascade consists of MEK kinase (Raf-1 or B-Raf), MAP/ERK kinase (MEK1 or MEK2), and extracellular-regulated protein kinase (ERK1 or ERK2). Raf becomes activated following its interaction with Ras-GTP (activated Ras). Via phosphorylation reactions, Raf activates MEK which, in turn, activates ERK. MEK5, also known as MAP Kinase 5, is a 50 kDa protein that is 40% identical to MEK1 and MEK2. Alternative splicing results in two MEK5 isoforms: alpha (50 kDa) and beta (40 kDa). MEK5beta has been reported to be ubiquitously expressed while MEK5alpha is expressed primarily in the liver and brain. The N-terminal amino acid sequence of MEK5alpha resembles sequences found in actin cytoskeletal proteins, indicating that MEK5alpha may associate with the cytoskeleton. However, the role of MEK5 in the MAP kinase pathway and its substrate remain to be identified. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase-5
Molecular Weight 50 kDa
Research Area Cancer, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) Western blot analysis of MEK5 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TI...
Immunofluorescence (IF) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) Immunofluorescence staining on human endothelial cells.
 image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 13-188) antibody (Image 3)
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 13-188) antibody (Image 4)
Product cited in: Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995 (PubMed).

English, Vanderbilt, Xu et al.: "Isolation of MEK5 and differential expression of alternatively spliced forms." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28897-902, 1996 (PubMed).