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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody

Details for Product No. ABIN968275, Supplier: Log in to see
Antigen
  • AA959795
  • bHLHe78
  • hif-1a
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-alpha
  • HIF1-ALPHA
  • hif1a
  • HIF1alpha
  • MOP1
  • PASD8
Epitope
AA 610-727
84
58
39
39
20
18
15
14
14
13
13
13
13
10
8
8
6
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
584
263
203
118
85
68
61
56
50
50
38
36
22
19
18
17
12
12
1
1
1
1
1
1
1
1
Host
Mouse
378
277
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
51
33
32
26
25
25
17
17
14
14
12
12
6
6
6
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
497
222
205
190
177
157
102
92
49
15
12
11
11
10
7
7
4
4
3
3
2
2
1
1
Supplier
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Immunogen Human HIF-1alpha aa. 610-727
Clone 54-HIF
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name HIF-1alpha (HIF1A Antibody Abstract)
Background Molecular oxygen (O2) is essential for mammalian metabolic processes such as oxidative phosphorylation. Thus, survival depends upon instantaneous transcriptional modulation of genes that maintain O2 homeostasis. Transcriptional control of several of these genes is mediated by hypoxia-inducible factor 1 (HIF-1). HIF-1 is a heterodimer whose alpha and beta subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. Common structural features of these proteins are an N-terminal bHLH DNA-binding domain and multiple PAS domains that confer dimerization ability and target gene specificity. Members diverge in their C-terminal regions. HIF-1beta is also known as the arylhydrocarbon nuclear translocator which is part of the functional dioxin receptor. However, HIF-1alpha functions exclusively to mediate responses to O2 deprivation. It contains C-terminal and internal transactivation domains. Although HIF-1 aproteinlevels increase during hypoxia, it is unstable in the presence of O2 due to an oxygen-dependent degradation domain (ODD) that targets it for ubiquitination. Thus, HIF-1alpha is essential for functional HIF-1 to mediate gene transcription in response to hypoxia. This antibody is routinely tested by western blot analysis.
Molecular Weight 120 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (ABIN968275) Western blot analysis of HIF-1alpha on lysate from HeLa stimulated with CoCl2, cobalt...
 image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (ABIN968275) anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (Image 2)
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (ABIN968275) anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 610-727) antibody (Image 3)
Product cited in: Fallone, Britton, Nieto, Salles, Muller: "ATR controls cellular adaptation to hypoxia through positive regulation of hypoxia-inducible factor 1 (HIF-1) expression." in: Oncogene, Vol. 32, Issue 37, pp. 4387-96, 2013 (PubMed).

Eckard, Dai, Wu, Jian, Yang, Chen, Costa, Frenkel, Huang: "Effects of cellular iron deficiency on the formation of vascular endothelial growth factor and angiogenesis. Iron deficiency and angiogenesis." in: Cancer cell international, Vol. 10, pp. 28, 2010 (PubMed).

Arsham, Plas, Thompson, Simon: "Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15162-70, 2002 (PubMed).

Sánchez-Elsner, Botella, Velasco, Langa, Bernabéu: "Endoglin expression is regulated by transcriptional cooperation between the hypoxia and transforming growth factor-beta pathways." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43799-808, 2002 (PubMed).

Wiesener, Münchenhagen, Berger, Morgan, Roigas, Schwiertz, Jürgensen, Gruber, Maxwell, Löning, Frei, Maher, Gröne, Eckardt: "Constitutive activation of hypoxia-inducible genes related to overexpression of hypoxia-inducible factor-1alpha in clear cell renal carcinomas." in: Cancer research, Vol. 61, Issue 13, pp. 5215-22, 2001 (PubMed).

Srinivas, Leshchinsky, Sang, King, Minchenko, Caro: "Oxygen sensing and HIF-1 activation does not require an active mitochondrial respiratory chain electron-transfer pathway." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21995-8, 2001 (PubMed).

Background publications Wang, Jiang, Rue, Semenza: "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5510-4, 1995 (PubMed).