Protein Disulfide Isomerase Family A, Member 4 (PDIA4) (AA 427-642) antibody

Antigen: Protein Disulfide Isomerase Family A, Member 4 (PDIA4)

Synonyms: ERP70, ERP72, MGC137149, PDIA4, LOC100229535

Binding Site: AA 427-642

Clonality: Monoclonal (3)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968286

Additional Information

Alternative name: ERp72

Immunogen: Human ERp72

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 3

Description: Within the ER lumen, numerous molecular chaperones transiently bind nascent proteins and facilitate their folding or assembly. The action of chaperones ensures proper folding and, in turn, transport of new proteins from the rough ER to the Golgi complex. Some chaperones (grp78/Bip) bind to exposed hydrophobic segments, while others (ERp59/PDI) are involved in disulfide isomerization. An ERp59/PDI related protein, ERp72, contains three copies of the PDI acitve site, CGHC. Although ERp72 participates in disulfide isomerization, it is unclear whether it functions alone or in conjunction with other chaperones, specifically other members of the CGHC-containing protein family. Many chaperones, including PDI, are held within the ER via the common C-terminal KDEL sequence. ERp72 contains a related KEEL sequence that is essential for its ER retention. Additional studies have shown that ERp72 is a protease and a member of the thioredoxin superfamily known to bind Ca2+. Thus, ERp72 functions as a Ca2+-dependent chaperone with disulfide isomerization activity, but also may undertake other, yet undefined, roles within the ER. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 69 kDa

Comments:

Related Products: ABIN967389, ABIN968537

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of ERp72 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-ERp72 antibody.

Immunofluorescence staining of human endothelial cells.

anti-Protein Disulfide Isomerase Family A, Member 4 (PDIA4) (AA 427-642) antibody (Image 3)

Publications

Product: Mazzarella, Srinivasan, Haugejorden et al.: "ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase." in: The Journal of biological chemistry, Vol. 265, Issue 2, pp. 1094-101, 1990 (PubMed).

Kuznetsov, Chen, Nigam: "Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation." in: The Journal of biological chemistry, Vol. 269, Issue 37, pp. 22990-5, 1994 (PubMed).

Nigam, Goldberg, Ho et al.: "A set of endoplasmic reticulum proteins possessing properties of molecular chaperones includes Ca(2+)-binding proteins and members of the thioredoxin superfamily." in: The Journal of biological chemistry, Vol. 269, Issue 3, pp. 1744-9, 1994 (PubMed).

Daoudal, Tournaire, Halere et al.: "Isolation of the mouse aldose reductase promoter and identification of a tonicity-responsive element." in: The Journal of biological chemistry, Vol. 272, Issue 5, pp. 2615-9, 1997 (PubMed).