CHRNA1 antibody (AA 332-457)

Catalog No. ABIN968298

Product Details anti-CHRNA1 Antibody

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Binding Specificity: AA 332-457

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHRNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Acetylcholine Receptor alpha aa. 332-457

Clone: 26-Acetylcholine Receptor alpha

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CHRNA1 antibody (ABIN968298)

Kincer, Uittenbogaard, Dressman, Guerin, Febbraio, Guo, Smart: "Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23525-33, (2002) (PubMed).

Spencer, Guyon, Sorimachi, Potts, Richard, Herasse, Chamberlain, Dalkilic, Kunkel, Beckmann: "Stable expression of calpain 3 from a muscle transgene in vivo: immature muscle in transgenic mice suggests a role for calpain 3 in muscle maturation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 13, pp. 8874-9, (2002) (PubMed).

Garchon, Djabiri, Viard, Gajdos, Bach: "Involvement of human muscle acetylcholine receptor alpha-subunit gene (CHRNA) in susceptibility to myasthenia gravis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4668-72, (1994) (PubMed).

Schröder, Reinhardt-Maelicke, Schrattenholz, McLane, Kretschmer, Conti-Tronconi, Maelicke: "Monoclonal antibodies FK1 and WF6 define two neighboring ligand binding sites on Torpedo acetylcholine receptor alpha-polypeptide." in: The Journal of biological chemistry, Vol. 269, Issue 14, pp. 10407-16, (1994) (PubMed).

Tsim, Greenberg, Rimer, Randall, Salpeter: "Transcripts for the acetylcholine receptor and acetylcholine esterase show distribution differences in cultured chick muscle cells." in: The Journal of cell biology, Vol. 118, Issue 5, pp. 1201-12, (1992) (PubMed).

Target Details for CHRNA1

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Alternative Name: Acetylcholine Receptor alpha (CHRNA1 Products)

Synonyms: CHRNA1 antibody, ACHRA antibody, ACHRD antibody, CHRNA antibody, CMS2A antibody, FCCMS antibody, SCCMS antibody, NACHRA1 antibody, AI385656 antibody, AI608266 antibody, Achr-1 antibody, Acra antibody, cholinergic receptor nicotinic alpha 1 subunit antibody, cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) antibody, CHRNA1 antibody, Chrna1 antibody

Background: Acetylcholine is an amine neurotransmitter at the neuromuscular junction. It is released from the presynaptic membrane of a cholinergic synapse into the synaptic cleft. It diffuses across the cleft and binds acetylcholine receptors (AChR) on the postsynaptic membrane. Receptor binding induces postsynaptic membrane depolarization and the generation of an action potential that produces effects such as muscle contraction. The AChR is a 250kDa pentameric complex of four transmembrane subunits in a stoichiometry of alpha2betagammadelta. In response to ligand binding, all subunits participate in the formation of an integral cation channel. However, the acetylcholine binding site is primarily within the alpha subunit. Myasthenia gravis (MG) is an autoimmune condition in which AchR levels are decreased. Autoantibodies bind and crosslink the AchRs leading to their internalization and degradation. This results in a decreased number of functional AChRs. Patients develop muscular weakness and some voluntary muscle fatigue. However, development of MG is also affected by genetic factors. One of the allelic forms of the AChRalpha gene appears to significantly contribute to MG susceptibility.

Molecular Weight: 49 kDa

Pathways: Skeletal Muscle Fiber Development