AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 460-781)

Details for Product anti-AKAP8 Antibody No. ABIN968304, Supplier: Log in to see
Antigen
  • MGC108502
  • AKAP8
  • AKAP 95
  • AKAP-8
  • AKAP-95
  • AKAP95
  • 1200016A02Rik
  • AA673585
  • AU015639
  • Akap95
  • A kinase (PRKA) anchor protein 8
  • AKAP8
  • akap8
  • Akap8
Epitope
AA 460-781
12
8
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
90
41
38
12
6
4
3
2
1
1
1
Host
Mouse
59
16
16
Clonality (Clone)
Monoclonal ()
Conjugate
This AKAP8 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
73
28
26
19
16
13
7
7
4
3
2
1
1
Options
Supplier
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Immunogen Human AKAP95 aa. 460-781
Clone 47-AKAP95
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name AKAP95 (AKAP8 Antibody Abstract)
Background Induction of cAMP results in the activation of the cAMP-dependent protein kinase (PKA) which, in turn, phosphorylates and regulates downstream effectors. The localization and, hence, the specificity of PKA action is mediated by the interaction of four different PKA regulatory subunits (RIalpha, RIbeta, RIIalpha, and RIIbeta) and specific PKA anchoring proteins. Several proteins have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Several AKAPs (AKAP79/75, AKAP350, and AKAP250) associate with PKA type II and direct its localization to cortical actin, centrosomes, and filopodia. AKAP95 is a specific nuclear anchoring protein that contains two zinc finger motifs and an RII binding domain. Although AKAP95 does not interact with RIIbeta, it has a high affinity for RIIalpha. During interphase, AKAP95 is bound to DNA. However, entry into mitosis results in AKAP95 detachment from the DNA and association with RIIalpha. Although the specific function of this AKAP95/RIIalpha heterodimer is unknown, its formation is cell cycle-dependent and may be essential for proper progression through the cell cycle.
Molecular Weight 95 kDa
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 460-781) (ABIN968304) Western blot analysis of AKAP95 on Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, lane ...
Immunofluorescence (IF) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 460-781) (ABIN968304) immunofluorescence staining on FHs 738 (ATCC HTB-157) cells.
 image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 460-781) (ABIN968304) anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 460-781) antibody (Image 3)
Western Blotting (WB) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 460-781) (ABIN968304) anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 460-781) antibody (Image 4)
Product cited in: Schillace, Andrews, Liberty, Davey, Carr: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, 2002 (PubMed).

Collas, Le Guellec, Taskén: "The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1167-80, 2000 (PubMed).

Steen, Cubizolles, Le Guellec, Collas: "A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract." in: The Journal of cell biology, Vol. 149, Issue 3, pp. 531-6, 2000 (PubMed).

Steen, Martins, Taskén, Collas: "Recruitment of protein phosphatase 1 to the nuclear envelope by A-kinase anchoring protein AKAP149 is a prerequisite for nuclear lamina assembly." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1251-62, 2000 (PubMed).

Background publications Eide, Coghlan, Orstavik, Holsve, Solberg, Skâlhegg, Lamb, Langeberg, Fernandez, Scott, Jahnsen, Taskén: "Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95." in: Experimental cell research, Vol. 238, Issue 2, pp. 305-16, 1998 (PubMed).