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Unc-13 Homolog B (C. Elegans) (UNC13B) (AA 621-834) antibody

Details for Product No. ABIN968305
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Antigen
Synonyms Unc13h2, Munc13-2, MUNC13, UNC13, UNC13B
Epitope
AA 621-834
(14), (7), (7), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(36), (21), (13), (13), (12), (12), (12)
Host
Mouse
(39), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(31), (25), (10), (5), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN968305
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Immunogen Rat Munc13-1
Clone TRAP-01
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Munc13 (Pan)
Background Release of neurotransmitters at the synaptic junction is a primary mechanism of neuronal communication and is regulated by the synaptic vesicle (SV) cycle. Several components of the SV cycle were initially characterized in C. elegans. One of those, unc-13, is involved in normal presynaptic function. Mammalian homologues of unc-13 are Munc13-1, 13-2, and 13-3. These neuron-specific proteins lack N-terminal homology, but are highly conserved in their C-terminal regions. Here they contain a phorbal ester binding C1-domain and two C2-domains with homology to the PKC Ca2+/phospholipid-binding domain. The most abundant isoform, Munc 13-1, is a presynaptic receptor with high affinities for phorbol ester and DAG. In response to phorbol ester, Munc13-1 associates with the plasma membrane and acts as a stimulation dependent enhancer of neurotransmitter release. In addition, Munc13-1 interacts with components of the SV cycle (syntaxin, SNAP25, synaptobrevin, Doc2) and with a brain specific isoform of beta-spectrin, a protein that interacts with the actin cytoskeleton. Thus, it is thought that Munc13 proteins function in signaling pathways that regulate the neuronal exocytic machinery.
Molecular Weight 196 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Unc-13 Homolog B (C. Elegans) (UNC13B) (AA 621-834) antibody Western blot analysis of panMunc13 on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of panMunc13.
Product cited in: Brose, Hofmann, Hata et al.: "Mammalian homologues of Caenorhabditis elegans unc-13 gene define novel family of C2-domain proteins." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25273-80, 1995 (PubMed).

Orita, Naito, Sakaguchi et al.: "Physical and functional interactions of Doc2 and Munc13 in Ca2+-dependent exocytotic machinery." in: The Journal of biological chemistry, Vol. 272, Issue 26, pp. 16081-4, 1997 (PubMed).

Betz, Ashery, Rickmann et al.: "Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release." in: Neuron, Vol. 21, Issue 1, pp. 123-36, 1998 (PubMed).

Sakaguchi, Orita, Naito et al.: "A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13." in: Biochemical and biophysical research communications, Vol. 248, Issue 3, pp. 846-51, 1998 (PubMed).

General Sowa, Orita, Minamikawa et al.: "Histone deacetylase inhibitor activates the WAF1/Cip1 gene promoter through the Sp1 sites." in: Biochemical and biophysical research communications, Vol. 241, Issue 1, pp. 142-50, 1998 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968305
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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