Transcription Elongation Regulator 1 (TCERG1) (AA 568-678) antibody

Details for Product No. ABIN968310
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Antigen
Synonyms CA150, TAF2S, Urn1, p144, FBP23, FBP28, Taf2s, ca150, CA150b, AI428505, 2410022J09Rik, 2900090C16Rik, tcerg1, wu:fb80g01, ATPRP40C, pre-mRNA-processing protein 40C
Epitope
AA 568-678
(2), (2), (2), (1), (1), (1)
Reactivity
Human
(27), (15), (14), (14), (12), (12), (12), (1)
Host
Mouse
(23), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(17), (10), (7), (5), (4), (3), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN968310
Quantity 50 µg
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Immunogen Human CA150
Clone 5
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CA150
Background Human immunodeficiency virus type 1 (HIV-1) promoter activation requires the binding of Tat (transactivator protein) and other coactivator proteins to viral RNA elements such as TAR (transactivation response element). Interactions between Tat and host factors may serve to regulate HIV-1 transcription. CA150 (Coactivator 150 kDa), a nuclear protein that associates with human RNA polymerase II holoenzyme, is a transcriptional coactivator of Tat. CA150 contains a glutamine and alanine rich repeat (QA), a feature of transcriptional activators, and proline rich and WWP regions that function in protein-protein interactions. In addition, CA150 contains a putative leucine zipper and a nuclear localization sequence (NLS). In vitro depletion of CA150 abrogated Tat mediated transactivation, while over-expression of a CA150 mutant lowered Tat-mediated activation in vivo. In addition, both CA150 and Tat coimmunoprecipitate with RNA polymerase II holoenzyme. These results suggest that CA150 is a transcriptional coactivator that associates with RNA polymerase II holoenzyme and, in turn, is involved in Tat-mediated transcription of the HIV-1 promoter.
Synonyms: Coactivator 150 kDa
Molecular Weight 150 kDa
Research Area Cancer, Transcription Factors
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Transcription Elongation Regulator 1 (TCERG1) (AA 568-678) antibody Western blot analysis of CA150 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-CA150 antibody.
anti-Transcription Elongation Regulator 1 (TCERG1) (AA 568-678) antibody (2) Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
anti-Transcription Elongation Regulator 1 (TCERG1) (AA 568-678) antibody (3) anti-Transcription Elongation Regulator 1 (TCERG1) (AA 568-678) antibody (Image 3)
Product cited in: Suñé, Hayashi, Liu et al.: "CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6029-39, 1997 (PubMed).

Hosts (23), (4)
Reactivities (27), (15), (14), (14), (12), (12), (12), (1)
Applications (17), (10), (7), (5), (4), (3), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (1), (1), (1)
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