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Integrin alpha 2 (ITGA2) (AA 42-245) antibody

Details for Product No. ABIN968318, Supplier: Log in to see
Antigen
  • BR
  • CD49B
  • DX5
  • gpi
  • GPIa
  • HPA-5
  • PGI
  • pgi-1
  • VLA-2
  • VLAA2
  • wu:fc02c05
  • zgc:86919
Epitope
AA 42-245
37
16
16
14
8
6
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
222
123
11
5
5
5
5
2
1
1
1
1
Host
Mouse
135
86
84
19
7
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
46
37
31
13
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
223
79
67
45
38
29
26
24
11
9
6
5
5
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Human VLA-2alpha aa.42-245
Clone 2-CD49b
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD49b (ITGA2 Antibody Abstract)
Background Integrins are a family of dimeric proteins that mediate cell-to-cell and extracellular matrix adhesion. They consist of a large alpha chain that is non-covalently associated with a smaller beta chain which is used to define integrin subfamilies. These molecules exhibit a wide range of expression throughout development and adulthood. VLA-2 (very late antigen), a member of the integrin superfamily, was identified on activated T cells, but has since been reported to be on various cell types. VLA-2 is reported to be a heterodimer of integrin alpha2 (CD49b) and integrin beta1 (CD29) subunits. The alpha2 chain contains a large extracellular domain, a transmembrane domain, and a short cytoplasmic tail. VLA-2 functions as a collagen receptor on platelets and fibroblasts, as well as a collagen and laminin receptor on endothelial and epithelial cells. On activated T cells, VLA-2, like LFA-1, exhibits increased number and affinity of ligand binding. Interactions of these molecules with their extracellular matrix ligands is important for directing effector T cells to their target tissues and to provide co-stimulatory signals. Thus, VLA-2 not only plays an important role in cellular adhesion, but may function in intracellular signal transmission.
Synonyms: Integrin alpha2, VLA-2alpha
Molecular Weight 150 kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (ABIN968318) Western blot analysis of CD49b (Integrin alpha2) on a HeLa cell lysate (Human cervica...
Immunofluorescence (IF) image for anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (ABIN968318) Immunofluorescence staining of WiDr cells (Human colorectal adenocarcinoma, ATCC CCL-...
 image for anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (ABIN968318) anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (Image 3)
Western Blotting (WB) image for anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (ABIN968318) anti-Integrin alpha 2 (ITGA2) (AA 42-245) antibody (Image 4)
Product cited in: Förster, Mäkela, Wärri, Kietz, Becker, Hultenby, Warner, Gustafsson: "Involvement of estrogen receptor beta in terminal differentiation of mammary gland epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 24, pp. 15578-83, 2002

Russell, Tofilon: "Radiation-induced activation of nuclear factor-kappaB involves selective degradation of plasma membrane-associated I(kappa)B(alpha)." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3431-40, 2002

Emsley, King, Bergelson, Liddington: "Crystal structure of the I domain from integrin alpha2beta1." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28512-7, 1997

Wu, Santoro: "Complex patterns of expression suggest extensive roles for the alpha 2 beta 1 integrin in murine development." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 199, Issue 4, pp. 292-314, 1994

Takada, Hemler: "The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain." in: The Journal of cell biology, Vol. 109, Issue 1, pp. 397-407, 1989