Protein Phosphatase 5, Catalytic Subunit (PPP5C) (AA 36-238) antibody

Details for Product No. ABIN968322
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Antigen
Synonyms pp5, MGC89961, fc83f08, im:7146608, wu:fc83f08, zgc:110801, PPP5C, PP5, PPP5, PPT, AU020526, REF1, TFPI-2
Epitope
AA 36-238
(8), (5), (4), (3), (2), (2), (1), (1)
Reactivity
Rat (Rattus)
(43), (6), (4)
Host
Mouse
(30), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(40), (17), (13), (10), (8), (5), (4), (4), (1)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN968322
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Immunogen Rat PP5
Clone 3
Isotype IgG1
Cross-Reactivity Mouse (Murine), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PP5
Background Phosphorylation and dephosphorylation on serine and threonine residues is critical for signal transduction and the regulation of numerous cellular functions. Phosphorylation levels are modulated by protein kinases and phosphatases. The Ser/Thr phosphatase PP5 (also known as PPT) is a model protein of the fourth subfamily of the PPP-family of phosphatases which includes PP1, PP2A, and PP2B. PP5 is primarily located in the nucleus, demonstrates widespread tissue distribution, and is highly conserved among species. It consists of a C-terminal catalytic domain and an N-terminal TPR (tetratricopeptide repeat) domain which is involved in protein-protein interactions. The TPRs of PP5 mediate protein phosphatase activity. TPRs mediate the interaction of PP5 with various proteins including the hsp90-glucocorticoid receptor complex and the kinase domain of ANP-guanylate cyclase receptor. PP5 is thought to be important in cell growth. For example, it functions upstream of p53 to regulate p21/WAF1/Cip1-mediated G1 growth arrest. Thus, PP5 is a nuclear protein phosphatase that is involved in signaling pathways of cell growth and arrest. This antibody is routinely tested by western blot analysis.
Synonyms: PPT
Molecular Weight 58 kDa
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Kinases/Phosphatases, DNA/RNA
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Protein Phosphatase 5, Catalytic Subunit (PPP5C) (AA 36-238) antibody Immunofluorescence staining of MDCK cells (Canine kidney, ATCC CCL-34).
anti-Protein Phosphatase 5, Catalytic Subunit (PPP5C) (AA 36-238) antibody (2) Western blot analysis of PP5 on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-PP5 antibody.
anti-Protein Phosphatase 5, Catalytic Subunit (PPP5C) (AA 36-238) antibody (3) anti-Protein Phosphatase 5, Catalytic Subunit (PPP5C) (AA 36-238) antibody (Image 3)
Product cited in: Chen, McPartlin, Brown et al.: "A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus." in: The EMBO journal, Vol. 13, Issue 18, pp. 4278-90, 1994 (PubMed).

Becker, Kentrup, Klumpp et al.: "Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain." in: The Journal of biological chemistry, Vol. 269, Issue 36, pp. 22586-92, 1994 (PubMed).

Ollendorff, Donoghue: "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32011-8, 1998 (PubMed).

Das, Cohen, Barford: "The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions." in: The EMBO journal, Vol. 17, Issue 5, pp. 1192-9, 1998 (PubMed).

Zuo, Dean, Honkanen: "Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21(WAF1/Cip1) and mediate growth arrest." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12250-8, 1998 (PubMed).

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