Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1) (AA 268-377) antibody

Details for Product No. ABIN968328
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Antigen
Synonyms D9S411E, LIN10, MINT1, X11, X11A, X11ALPHA, Mint1, Mint, Lin-10, Urop11, X11alpha, 6430513E09Rik, APBA1
Epitope
AA 268-377
(15), (6), (5), (2), (1), (1)
Reactivity
Rat (Rattus)
(50), (38), (34), (12), (12), (12), (12)
Host
Mouse
(52), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(43), (33), (12), (10), (7), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN968328
Quantity 150 µg
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Immunogen Rat Mint 1
Clone 23
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Mint1
Background Neuronal communication via neurotransmitter release is mediated by the synaptic vesicle cycle. The initial step in exocytosis is the docking of the vesicle in the active zone of the plasma membrane. This step is followed by fusion of the vesicle and plasma membrane and exocytosis. Munc18-1, a major brain protein, is essential for exocytosis. It binds the vesicle fusion protein syntaxin, along with Doc2a and 2b, two proteins that associate peripherally with the synaptic vesicle. Munc18-1 is a family member of membrane trafficking proteins. Its function is thought to be mediated by two Munc18-1-interacting proteins termed Mint 1 and Mint 2, which are 50% homologous. They are expressed exclusively in brain and bind Munc18-1 (MID) with high affinity. They contain an N-terminal Munc18-1 interacting domain and C-terminal PTB (pTyr/PIP interaction) and PDZ (membrane protein interaction) domains, suggesting the ability of Mint proteins to link vesicle exocytosis to Tyr phosphorylation and/or localization at synaptic intercellular junctions. Thus, the Mint proteins, along with Munc18-1 and syntaxin, form a multimeric complex that mediates appropriate docking/fusion of synaptic vesicles. This antibody is routinely tested by western blot analysis.
Molecular Weight 120 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1) (AA 268-377) antibody Western blot analysis of Mint1 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Mint1 antibody.
anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1) (AA 268-377) antibody (2) Immunofluorescence staining of rat neurons.
anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 1 (APBA1) (AA 268-377) antibody (3) Immunohistochemical staining of a formalin-fixed, paraffin embedded rat cerebrum tissue section, with citrate pre-treatment (20X magnification).
Product cited in: Okamoto, Südhof: "Mints, Munc18-interacting proteins in synaptic vesicle exocytosis." in: The Journal of biological chemistry, Vol. 272, Issue 50, pp. 31459-64, 1998 (PubMed).

Biederer, Südhof: "Mints as adaptors. Direct binding to neurexins and recruitment of munc18." in: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 39803-6, 2001 (PubMed).

Fisher, Pevsner, Burgoyne: "Control of fusion pore dynamics during exocytosis by Munc18." in: Science (New York, N.Y.), Vol. 291, Issue 5505, pp. 875-8, 2001 (PubMed).

Wong, Setou, Teng et al.: "Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 22, pp. 14500-5, 2002 (PubMed).

Hosts (52), (5), (1)
Reactivities (50), (38), (34), (12), (12), (12), (12)
Applications (43), (33), (12), (10), (7), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (6), (5), (2), (1), (1)
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