DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 105-305) antibody

Details for Product No. ABIN968331
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Antigen
Synonyms DFFA, DFF-45, DFF1, ICAD, A330085O09Rik, DFF35, Dff45, ICAD-L, ICAD-S
Epitope
AA 105-305
(43), (35), (14), (9), (8), (7), (6), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(140), (74), (22), (2)
Host
Mouse
(174), (22), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(167), (73), (30), (30), (17), (17), (15), (10), (7), (4), (2), (1)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN968331
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Immunogen Human DFF45
Clone Hs-3
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name DFF45
Background Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is the most extensively studied apoptotic protein. An apoptotic signal induces the intracellular cleavage of caspase-3 from an inactive proform to an active fragment. The active form of caspase-3 cleaves several other apoptotic proteins including DNA Fragmentation Factor (DFF). DFF is thought to be either directly or indirectly responsible for the DNA laddering that is a hallmark of apoptosis. It is a heterodimer of 40 kDa (DFF40) and 45 kDa (DFF45) subunits. Cleavage of DFF45 into three fragments is a prerequisite for DFF-mediated DNA fragmentation. Identity of the DFF fragment that mediates DNA laddering remains unclear. However, it is clear that DFF functions downstream of caspase-3 and is essential for the characteristic fragmentation of DNA during apoptosis.
Synonyms: DNA Fragmentation Factor-45
Molecular Weight 45 kDa
Research Area Apoptosis/Necrosis, DNA/RNA
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 105-305) antibody Western blot analysis of DFF45 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152) . Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti-human DFF45 antibody.
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 105-305) antibody (2) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 105-305) antibody (Image 2)
Product cited in: Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).

Inohara, Koseki, Chen et al.: "CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor." in: The EMBO journal, Vol. 17, Issue 9, pp. 2526-33, 1998 (PubMed).

Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).

Fulda, Meyer, Debatin: "Inhibition of TRAIL-induced apoptosis by Bcl-2 overexpression." in: Oncogene, Vol. 21, Issue 15, pp. 2283-94, 2002 (PubMed).

Li, Chen, Sinogeeva et al.: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, 2002 (PubMed).

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