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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 25-145)

Details for Product anti-CASP3 Antibody No. ABIN968341, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
Alternatives
anti-Mouse (Murine) Caspase 3 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 25-145
37
31
30
30
19
19
18
17
14
13
13
12
12
10
9
8
7
6
6
6
5
5
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4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
451
188
137
27
17
14
13
13
12
9
6
5
5
4
3
2
1
Host
Mouse
441
65
16
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 3 antibody is un-conjugated
24
21
17
10
10
9
4
3
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
365
193
160
87
62
58
37
24
23
21
13
10
7
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Mouse CPP32 aa. 25-145
Clone 46-Caspase
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is a member of the family of cysteine proteases which includes interleukin-1beta-converting enzyme (ICE) and C. elegans protein, Ced-3. An apoptotic signal such as granzyme B of cytotoxic T-cells (CTLs) or ICE-like proteases induces the intracellular cleavage of Caspase-3 from the inactive proform (32kDa) to the active form which consists of the p20, p17, and p12 subunits. The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA fragmentation factor (DFF). Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. This antibody recognizes the mouse 32 kDa pro-Caspase-3 and the p17 cleaved form of Caspase 3 in T cell lymphocytes treated with camptothecin.
Synonyms: CPP32
Molecular Weight 32 kDa
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 25-145) (ABIN968341) Western blot analysis of Caspase-3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000,...
Immunofluorescence (IF) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 25-145) (ABIN968341) Immunofluorescence staining of RSV-3T3 cells.
Product cited in: Li, Zhao, He et al.: "Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26912-20, 2002 (PubMed).

Jia, Patwari, Srinivasula et al.: "Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis." in: Oncogene, Vol. 20, Issue 35, pp. 4817-26, 2001 (PubMed).

Hatai, Matsuzawa, Inoshita et al.: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, 2000 (PubMed).

Background publications Liu, Kim, Yang et al.: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 (PubMed).

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).