Eukaryotic Translation Initiation Factor 5 (EIF5) (AA 100-215) antibody
|Synonyms||EIF-5A, MGC36374, MGC36509, D12Ertd549e, 2810011H21Rik, fb37c12, fb54h04, zgc:56606, zgc:77026, wu:fb37c12, wu:fb54h04|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|5 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs). As a prerequisite to initiation, the initiator methionyl-tRNA (Met-tRNAf), in a ternary complex with eIF-2 and GTP, binds to the 40S ribosomal subunit. This 40S preinitiation complex is positioned at the initiation AUG codon to form the 40S initiation complex. This complex is joined by the 60S ribosomal subunit to form the 80S initiation complex, which functions as a peptidyl transferase. The joining of the 60S subunit to the 40S initiation complex requires eIF-5. This protein associates with the 40S initiation complex, specifically with the beta subunit of eIF-2, prior to binding of the 60S subunit. eIF-5 induces GTP hydrolysis of the ternary complex bound to the 40S ribosome. This results in the release of eIF-2 and GDP from the 40S subunit and allows for interaction with the 60S subunit. Furthermore, eIF-5 is thought to be involved in recognition of the start codon and interacts with eIF-3, an additional factor required for 40S initiation complex formation. Thus, eIF-5 is essential to the assembly and proper function of the eukaryotic machinery. eIF-5 has a calculated molecular weight of 49 kDa, but has been reported to migrate in a range between 49-53 kDa.
Synonyms: Eukaryotic Initiation Factor-5
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||49-53 kDa|
Related Products: ABIN968587, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Chevesich, Chaudhuri, Maitra: "Characterization of mammalian translation initiation factor 5 (eIF-5). Demonstration that eIF-5 is a phosphoprotein and is present in cells as a single molecular form of apparent M(r) 58,000." in: The Journal of biological chemistry, Vol. 268, Issue 27, pp. 20659-67, 1993 (PubMed).
Si, Das, Maitra: "Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5)." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16934-8, 1996 (PubMed).
Das, Maiti, Das et al.: "Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the beta-subunit of eIF2." in: The Journal of biological chemistry, Vol. 272, Issue 50, pp. 31712-8, 1998 (PubMed).
Phan, Zhang, Asano et al.: "Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4935-46, 1998 (PubMed).
Kedersha, Chen, Gilks et al.: "Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 195-210, 2002 (PubMed).
|Hosts||Rabbit (41), Mouse (2)|
|Reactivities||Human (41), Rat (Rattus) (39), Mouse (Murine) (37), Cow (Bovine) (19), Horse (Equine) (18), Rabbit (18), Chicken (1), Dog (Canine) (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Immunofluorescence (IF) (32), Western Blotting (WB) (13), ELISA (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (2), N-Term (1)|