TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody
|Synonyms||MOT1, TAF172, KIAA0940, TAFII170, MGC138406, TAF(II)170|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|1 reference available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
TATA binding protein (TBP), along with several TBP-Associated Factors (TAF), is essential for eukaryotic transcription complex assembly. TBP-TAF complexes, such as SL1, TFIID, and TFIIB, direct the assembly of RNA polymerase (pol) I, II, and III transcription complexes, respectively. An additional TBP-TAF complex, SNAPc, is required for pol II and III mediated transcription of human snRNA genes. TAF-172 is an abundant human TBP-associated factor. It is homologous to the S. cerevisiae Mot1 ATPase which regulates transcription both negatively and positively. Both proteins are members of the Snf2/Swi2 family of DNA-targeted ATPases. Like Mot1, TAF-172 binds TBP and, via ATP hydrolysis, dissociates TBP from DNA. This hydrolysis also dissociates TAF-172 from TBP. The activity of TAF-172 requires DNA and TBP, and TAF-172 repression of TBP is reversed by TFIIA. In addition, TAF-172 inhibits TBP-driven, but not TBP-TAF-driven, pol II and III mediated transcription. Thus, TAF-172, the human homolog of yeast Mot1, functions as a positive or negative regulator of transcription by using the energy of ATP hydrolysis to target the removal of TBP from DNA.
Synonyms: TBP Associated Factor-172
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||172 kDa|
Related Products: ABIN968587, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Chicca, Auble, Pugh: "Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1701-10, 1998 (PubMed).
|Hosts||Rabbit (6), Mouse (3)|
|Applications||Western Blotting (WB) (7), Immunoprecipitation (IP) (6), ELISA (3), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunohistochemistry (IHC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1)|
|Epitopes||C-Term (2), AA 1050-1100 (1), AA 1325-1375 (1), AA 75-125 (1), N-Term (1)|