TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody

Details for Product No. ABIN968348
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Antigen
Synonyms MOT1, TAF(II)170, TAF172, TAFII170
Epitope
AA 71-193
(3), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(14)
Host
Mouse
(10), (4)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
(10), (10), (3), (3), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN968348
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Immunogen Human TAF-172
Clone 7
Isotype IgG1
Cross-Reactivity Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name TAF-172
Background TATA binding protein (TBP), along with several TBP-Associated Factors (TAF), is essential for eukaryotic transcription complex assembly. TBP-TAF complexes, such as SL1, TFIID, and TFIIB, direct the assembly of RNA polymerase (pol) I, II, and III transcription complexes, respectively. An additional TBP-TAF complex, SNAPc, is required for pol II and III mediated transcription of human snRNA genes. TAF-172 is an abundant human TBP-associated factor. It is homologous to the S. cerevisiae Mot1 ATPase which regulates transcription both negatively and positively. Both proteins are members of the Snf2/Swi2 family of DNA-targeted ATPases. Like Mot1, TAF-172 binds TBP and, via ATP hydrolysis, dissociates TBP from DNA. This hydrolysis also dissociates TAF-172 from TBP. The activity of TAF-172 requires DNA and TBP, and TAF-172 repression of TBP is reversed by TFIIA. In addition, TAF-172 inhibits TBP-driven, but not TBP-TAF-driven, pol II and III mediated transcription. Thus, TAF-172, the human homolog of yeast Mot1, functions as a positive or negative regulator of transcription by using the energy of ATP hydrolysis to target the removal of TBP from DNA.
Synonyms: TBP Associated Factor-172
Molecular Weight 172 kDa
Comment

Related Products: ABIN968587, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody Immunofluorescence staining of human endothelial cells.
anti-TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody (2) Western blot analysis of TAF-172 on a HepG2 cell lysate (Human hepatocellular carcinoma, ATCC HB-8065). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- TAF-172 antibody.
anti-TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody (3) anti-TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody (Image 3)
Product cited in: Chicca, Auble, Pugh: "Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1701-10, 1998 (PubMed).

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