TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody

Antigen: TBP-Associated Factor 172 (BTAF1)

Synonyms: MOT1, TAF172, KIAA0940, TAFII170, MGC138406, TAF(II)170

Binding Site: AA 71-193

Clonality: Monoclonal (7)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968348

Additional Information

Alternative name: TAF-172

Immunogen: Human TAF-172

Cross-Reactivity: Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 7

Description: TATA binding protein (TBP), along with several TBP-Associated Factors (TAF), is essential for eukaryotic transcription complex assembly. TBP-TAF complexes, such as SL1, TFIID, and TFIIB, direct the assembly of RNA polymerase (pol) I, II, and III transcription complexes, respectively. An additional TBP-TAF complex, SNAPc, is required for pol II and III mediated transcription of human snRNA genes. TAF-172 is an abundant human TBP-associated factor. It is homologous to the S. cerevisiae Mot1 ATPase which regulates transcription both negatively and positively. Both proteins are members of the Snf2/Swi2 family of DNA-targeted ATPases. Like Mot1, TAF-172 binds TBP and, via ATP hydrolysis, dissociates TBP from DNA. This hydrolysis also dissociates TAF-172 from TBP. The activity of TAF-172 requires DNA and TBP, and TAF-172 repression of TBP is reversed by TFIIA. In addition, TAF-172 inhibits TBP-driven, but not TBP-TAF-driven, pol II and III mediated transcription. Thus, TAF-172, the human homolog of yeast Mot1, functions as a positive or negative regulator of transcription by using the energy of ATP hydrolysis to target the removal of TBP from DNA.
Synonyms: TBP Associated Factor-172

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 172 kDa

Comments:

Related Products: ABIN968587, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of TAF-172 on a HepG2 cell lysate (Human hepatocellular carcinoma, ATCC HB-8065). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- TAF-172 antibody.

Immunofluorescence staining of human endothelial cells.

anti-TBP-Associated Factor 172 (BTAF1) (AA 71-193) antibody (Image 3)

Publications

Product: Chicca, Auble, Pugh: "Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1701-10, 1998 (PubMed).