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MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135)

Details for Product anti-MAP3K3 Antibody No. ABIN968366, Supplier: Login to see New
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Synonyms MAPKKK3, MEKK3, AW548911, Mekk3, mKIAA4031
AA 27-135
(17), (15), (11), (11), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(39), (39), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(57), (8)
Clonality (Clone)
Monoclonal ()
This MAP3K3 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Immunofluorescence (IF), Western Blotting (WB)
(60), (23), (12), (8), (6), (3), (2), (2), (1), (1)
Pubmed 2 references available
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Quantity 150 μg
Shipping to United States ( )
Immunogen Human MEKK3 aa. 27-135
Clone 40-MEKK3
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEKK3 (MAP3K3 Antibody Abstract)
Background The Ras signaling pathway links signals from growth factor receptors to activation of the MEK/ERK kinases. This cascade of phosphorylation leads to cell growth and differentiation. External stimuli such as endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression. These processes are controlled by the MKK3/p38MAP kinase cascade. A third pathway responsive to environmental stress, cell death, or apoptosis is regulated by SEK/SAPKs. When overexpresssed, MEKK3 is a kinase that is capable of activating both the ERK and SAPK cascades. MEKK3 is 626 amino acids long and is ubiquitously expressed. It is positioned upstream of SEK and MEK in the signaling pathways and directly phosphorylates these enzymes. Overexpression of MEKK3 results in the activation of NFkappaB. Thus, MEKK3 represents an emerging family of kinases that is capable of inducing more than one signaling pathway. This indicates that specificity in the signaling cascades is probably achieved by effector kinases such as the ERKs and SAPKs.
Molecular Weight 71 kDa
Research Area Immunology, Innate Immunity, Kinases/Phosphatases
Pathways MAPK Signaling

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135) (ABIN968366) Western blot analysis of MEKK3 on a HeLa cell lysate (Human cervical epitheloid carci...
Immunofluorescence (IF) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135) (ABIN968366) Immunofluorescence staining of RSV-3T3 cells.
 image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135) (ABIN968366) anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 27-135) antibody (Image 3)
Product cited in: Yang, Lai, Lai et al.: "Phenethyl isothiocyanate inhibits migration and invasion of human gastric cancer AGS cells through suppressing MAPK and NF-kappaB signal pathways." in: Anticancer research, Vol. 30, Issue 6, pp. 2135-43, 2010 (PubMed).

Ellinger-Ziegelbauer, Brown, Kelly et al.: "Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 ..." in: The Journal of biological chemistry, Vol. 272, Issue 5, pp. 2668-74, 1997 (PubMed).

Catalog No. ABIN968366
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