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MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290)

Details for Product anti-FRAP1 Antibody No. ABIN968383, Supplier: Log in to see
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
Alternatives
anti-Human MTOR antibody for Western Blotting
Epitope
AA 185-290
66
25
24
23
22
21
14
13
13
10
7
6
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
342
165
151
8
2
2
1
1
1
Host
Mouse
295
46
2
Clonality (Clone)
Monoclonal ()
Conjugate
This MTOR antibody is un-conjugated
8
7
7
5
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
222
112
92
63
51
45
32
20
8
8
6
3
2
2
2
2
2
1
Supplier
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Immunogen Rat RAFT1 aa. 185-290
Clone 30-RAFT1
Isotype IgG1
Cross-Reactivity Mouse (Murine), Human, Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name RAFT1 (FRAP1 Antibody Abstract)
Background Immunosuppressive agents such as cyclosporin A, FK506, and rapamycin are used routinely in clinical transplantation and the treatment of autoimmune disorders. Cyclosporin A and FK506 bind to cyclophilins and the FK-binding protein FKBP12, respectively. These complexes interact with, and inhibit the activity of, the calcineurin protein phosphatase, which is essential for T cell activation. Rapamycin also interacts with FKBP12, but this complex does not affect calcineurin. Instead, it inhibits signals required for G1 to S progression in yeast, in IL-2-stimulated T lymphocytes, and other mammalian cell types. Rapamycin-FKBP12 binds to a protein designated Rapamycin And FKBP12 Target-1 (RAFT1) in rats and FKBP-Rapamycin-Associated Protein (FRAP) in humans. RAFT1 is the homolog of the yeast genes TOR1 and TOR2 which, when mutated, lead to rapamycin resistance. RAFT1 is a PI4-kinase that exhibits homology with other PI kinases such as PI3-kinase and PI4-Kalpha. RAFT1 phosphorylates p70 S6 kinase and 4E-BP1 which are regulators of translational initiation. However, rapamycin-FKBP1 does not inhibit PI4-kinase activity and, therefore, affects RAFT1 activity via alternate mechanisms.
Synonyms: Rapamycin And FKBP12 Target-1
Molecular Weight 245 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-172...
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).
Product cited in: Castedo, Ferri, Blanco et al.: "Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation." in: The Journal of experimental medicine, Vol. 194, Issue 8, pp. 1097-110, 2001 (PubMed).

Alarcon, Cardenas, Heitman: "Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast." in: Genes & development, Vol. 10, Issue 3, pp. 279-88, 1996 (PubMed).

Sabatini, Pierchala, Barrow et al.: "The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20875-8, 1995 (PubMed).

Background publications Burnett, Barrow, Cohen et al.: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998 (PubMed).

Sabatini, Erdjument-Bromage, Lui et al.: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).