Period Homolog 2 (Drosophila) (PER2) (AA 1-112) antibody

Antigen: Period Homolog 2 (Drosophila) (PER2)

Synonyms: FASPS, KIAA0347

Binding Site: AA 1-112

Clonality: Monoclonal (52)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968388

Additional Information

Alternative name: Per2

Immunogen: Mouse Per2

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 52

Description: Circadian rhythms are behavioral or physiological rhythms with periods of approximately 24 hours. They have been identified in many species from bacteria to humans. In mammals, the circadian regulator is the suprachiasmatic nucleus (SCN) of the anterior hypothalamus. Multiple genes involved in the SCN circadian rhythm have been isolated. The Drosophila period (dper) gene is the most completely characterized mammalian clock gene. In mouse, multiple per genes have been identified and constitute a mammalian period gene family (mper1, mper2, and mper3). These proteins contain a dimerization PAS domain and a cytoplasmic localization domain. SCN expression of mper1 and mper2 overlaps, but is not synchronous. Although not light-inducible, mper2 transcripts are detected approximately two hours following those of mper1. This suggests that the mper1 gene product, a putative transcription factor, is produced in response to light and promotes the expression of mper2. Although mper1 and mper2 are similar, they are not redundant. Thus, these different circadian regulators may allow integration of a wider range of input signals and interact with different downstream effectors.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 105 kDa

Comments:

Related Products: ABIN967389, ABIN968545

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Per2 on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of Per2.

Immunofluorescence staining of Rat Neurons.

anti-Period Homolog 2 (Drosophila) (PER2) (AA 1-112) antibody (Image 3)

Publications

Product: Albrecht, Sun, Eichele et al.: "A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light." in: Cell, Vol. 91, Issue 7, pp. 1055-64, 1998 (PubMed).

Takumi, Taguchi, Miyake et al.: "A light-independent oscillatory gene mPer3 in mouse SCN and OVLT." in: The EMBO journal, Vol. 17, Issue 16, pp. 4753-9, 1998 (PubMed).