Stathmin 1 (STMN1) (AA 38-147) antibody

Details for Product No. ABIN968392
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Antigen
Synonyms
C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, 19k, P18, P19, Pig, Smn, Op18, Pp17, Pp18, Pp19, Pr22, Lap18, prosolin, op18, stathmin, stmn1, stmn1a, lag, lap18, pp17, pp19, pr22, smn, fj43c10, wu ... show more
C1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, 19k, P18, P19, Pig, Smn, Op18, Pp17, Pp18, Pp19, Pr22, Lap18, prosolin, op18, stathmin, stmn1, stmn1a, lag, lap18, pp17, pp19, pr22, smn, fj43c10, wu:fj38b07, wu:fj43c10, zgc:110159, cb959, wu:fb14e04, zgc:136942, LOC100217988 show less
Epitope
AA 38-147
(34), (23), (21), (20), (19), (17), (16), (16), (16), (12), (11), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(312), (235), (217), (53), (34), (1)
Host
Mouse
(304), (7), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), BioImaging (BI)
(225), (133), (98), (94), (60), (39), (32), (20), (4), (2), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN968392
Quantity 50 µg
Price
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Immunogen Human Metablastin
Clone FB11
Isotype IgG2b
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Metablastin
Background The regulation of microtubule (MT) assembly is vital to cellular processes such as organelle transport, organization of the cytoplasm, and intracellular movement of cell surface receptors. MTs are composed of tubulin subunits that exist in dynamic equilibrium between free tubulin dimers and MTs. The instability of MTs is determined by the rates of growth and shrinkage of tubulin polymers and by frequencies of transitions from growth to shrinkage (catastrophes) or from shrinkage to growth (rescues). The most well known MT regulators are the microtubule associated proteins (MAPs) which directly bind and stabilize MTs. Metablastin (stathmin) opposes MAP activity by inducing catastrophes.Metablastin is variably phosphorylated on multiple Ser residues by kinases that are regulated by the cell cycle or by external signals. Phosphorylation of metablastin inhibits its ability to destabilize MTs and, in turn, induces tubulin polymerization. Metablastin activity is turned off during the cell cycle to allow spindle formation and cell division. Thus, metablastin is thought to function to regulate the dynamics of MT formation in response to external signals during the interphase of the cell cycle.
Synonyms: Stathmin, Metablastin
Molecular Weight 19 kDa
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Stathmin 1 (STMN1) (AA 38-147) antibody Western blot analysis of Metablastin on a Jurkat lysate (ABIN968537). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Metablastin (Stathmin) antibody.
anti-Stathmin 1 (STMN1) (AA 38-147) antibody (2) Immunofluorescent staining of HeLa cells (ATCC CCL-2). Cells were seeded in a 96 well imaging plate at ~ 10 000 cells per well. After overnight incubation, cells were stained using the Triton™ X-100 perm protocol and the anti- Metablastin antibody. The second step reagent was FITC goat anti mouse Ig. The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells and can be used with either perm protocol.
anti-Stathmin 1 (STMN1) (AA 38-147) antibody (3) anti-Stathmin 1 (STMN1) (AA 38-147) antibody (Image 3)
Product cited in: Horwitz, Shen, He et al.: "The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8129-32, 1997 (PubMed).

Larsson, Marklund, Gradin et al.: "Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5530-9, 1997 (PubMed).

Gradin, Larsson, Marklund et al.: "Regulation of microtubule dynamics by extracellular signals: cAMP-dependent protein kinase switches off the activity of oncoprotein 18 in intact cells." in: The Journal of cell biology, Vol. 140, Issue 1, pp. 131-41, 1998 (PubMed).

Hosts (304), (7), (6)
Reactivities (312), (235), (217), (53), (34), (1)
Applications (225), (133), (98), (94), (60), (39), (32), (20), (4), (2), (1), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2)
Epitopes (34), (23), (21), (20), (19), (17), (16), (16), (16), (12), (11), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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