Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C) (AA 479-580) antibody

Antigen: Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C)

Synonyms: LCCS3, KIAA0589, PIPKIg_v4, PIP5Kgamma, PIP5K-GAMMA, MGC124518, MGC130766, PIP5K1C, MGC110576, zgc:110576, si:dkey-29i9.1

Binding Site: AA 479-580

Clonality: Monoclonal (12)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968393

Additional Information

Alternative name: PIP5K gamma

Immunogen: Mouse PIP5Kgamma

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 12

Description: Phosphoinositide turnover is a well established mechanism of intracellular signal transduction. Sequential phosphorylation of phosphatidylinositol (PtdIns) results in PtdIns(4)P (PIP) and PtdIns(4,5)P2 (PIP2). Phospholipase C (PLC) hydrolyzes PIP2 to inositol (1,4,5)P3 (IP3) which stimulates release of intracellular Ca2+. PIP2 is generated by phosphorylation of PtdIns 5-kinases (PI5-K). These enzymes are divided into two types (I and II) based on their size and sensitivity to certain compounds. Three mammalian PI5-Ks, PI5-Kalpha, beta, and gamma of type I have been identified and a type II PIP5kalpha. Although the PI4-Ks are abundantly distributed throughout the cell, activity is found primarily in association with membranous structures. Members of this family contain a lipid kinase unique domain and a C-terminal catalytic domain.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 87-90 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PIP5Kgamma on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-PIP5Kgamma antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).

anti-Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C) (AA 479-580) antibody (Image 3)

Publications

Product: Ishihara, Shibasaki, Kizuki et al.: "Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8741-8, 1998 (PubMed).

Tolias, Rameh, Ishihara et al.: "Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18040-6, 1998 (PubMed).

Nishikawa, Toker, Wong et al.: "Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type I phosphatidylinositol-4-phosphate 5-kinase." in: The Journal of biological chemistry, Vol. 273, Issue 36, pp. 23126-33, 1998 (PubMed).