Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12) (AA 1-119) antibody
|Synonyms||cb929, cb1094, zgc:66248, wu:fj94c07, Aralar1, RGD1561141|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|1 reference available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Description||Mitochondria are the primary sites of ATP production within the cell. They contain two different membranes, the outer membrane and the inner membrane. The outer membrane contains porins which form pores in the membrane and render it highly permeable. The inner membrane is less permeable and contains a high protein-to-lipid ratio. This membrane contains a number of carrier proteins that mediate metabolite transport. These belong to a superfamily of related proteins that are found in S. cerevisiae, C. elegans, and several mammalian species. Aralar, a human member of this family, is expressed in the mitochondria within heart, skeletal muscle, and brain. Its C-terminal half resembles proteins of the mitochondrial solute carrier superfamily and its N-terminal portion contains four putative Ca2+-binding EF-hand domains. The presence of the EF-hands in Aralar make it a close relative of the large family of Ca2+-binding proteins that includes calmodulin and myosin light chain. Aralar is thought to function as a Ca2+-regulated mitochondrial anion carrier or as a Ca2+ and anion cotransporter since it is expressed primarily in tissues where cell function relies heavily on Ca2+- signaling.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||70 kDa|
Related Products: ABIN968552, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
del Arco, Satrústegui: "Molecular cloning of Aralar, a new member of the mitochondrial carrier superfamily that binds calcium and is present in human muscle and brain." in: The Journal of biological chemistry, Vol. 273, Issue 36, pp. 23327-34, 1998 (PubMed).
|Reactivities||Human (23), Cow (Bovine) (19), Dog (Canine) (19), Mouse (Murine) (19), Rat (Rattus) (19), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (15), Western Blotting (WB) (8), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||Internal Region (2), N-Term (1)|