Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12) (AA 1-119) antibody

Antigen: Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12)

Synonyms: cb929, cb1094, zgc:66248, wu:fj94c07, Aralar1, RGD1561141

Binding Site: AA 1-119

Clonality: Monoclonal (8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968399

Additional Information

Alternative name: Aralar

Immunogen: Human Aralar

Format: Liquid

Isotype: IgG1

Clone: 8

Description: Mitochondria are the primary sites of ATP production within the cell. They contain two different membranes, the outer membrane and the inner membrane. The outer membrane contains porins which form pores in the membrane and render it highly permeable. The inner membrane is less permeable and contains a high protein-to-lipid ratio. This membrane contains a number of carrier proteins that mediate metabolite transport. These belong to a superfamily of related proteins that are found in S. cerevisiae, C. elegans, and several mammalian species. Aralar, a human member of this family, is expressed in the mitochondria within heart, skeletal muscle, and brain. Its C-terminal half resembles proteins of the mitochondrial solute carrier superfamily and its N-terminal portion contains four putative Ca2+-binding EF-hand domains. The presence of the EF-hands in Aralar make it a close relative of the large family of Ca2+-binding proteins that includes calmodulin and myosin light chain. Aralar is thought to function as a Ca2+-regulated mitochondrial anion carrier or as a Ca2+ and anion cotransporter since it is expressed primarily in tissues where cell function relies heavily on Ca2+- signaling.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 70 kDa

Comments:

Related Products: ABIN968552, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Aralar on a SW-13 cell lysate (Human adrenal gland carcinoma, ATCC CCL-105). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-human Aralar antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

anti-Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12) (AA 1-119) antibody (Image 3)

Publications

Product: del Arco, Satrústegui: "Molecular cloning of Aralar, a new member of the mitochondrial carrier superfamily that binds calcium and is present in human muscle and brain." in: The Journal of biological chemistry, Vol. 273, Issue 36, pp. 23327-34, 1998 (PubMed).