Golgi SNAP Receptor Complex Member 1 (GOSR1) (AA 3-108) antibody

Details for Product No. ABIN968404, Supplier: Log in to see
Antigen
  • CG7700
  • Dmel\\CG7700
  • FSG28
  • GOS28
  • dgos28
  • GOSR1
  • gosr1
  • zgc:112064
  • GOLIM2
  • GOS-28
  • GOS28/P28
  • GS28
  • P28
  • AI414660
  • AI426320
  • BB145494
  • GOSRI
  • p28
  • Gos28
  • v-SNARE
  • CG7700 gene product from transcript CG7700-RA
  • golgi SNAP receptor complex member 1
  • Golgi SNAP receptor complex member 1
  • Protein GOS-28
  • Gos28
  • GOSR1
  • gosr1
  • CpipJ_CPIJ014329
  • PAAG_02888
  • Tsp_07481
  • Gosr1
  • gos-28
Epitope
AA 3-108
7
5
4
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
47
33
30
6
6
6
5
4
4
3
2
2
2
1
1
1
Host
Mouse
38
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
38
13
11
9
7
5
3
2
2
1
Options
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Immunogen Rat GS28 aa. 3-108
Clone 1-GS28
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name GS28 (GOSR1 Antibody Abstract)
Background Most vesicular fusion events require the function of N-ethylmaleimide-sensitive factor (NSF) and the soluble NSF attachment proteins (SNAPs). Their function is dependent upon the SNAP receptor (SNARE) family of proteins. The specificity of vesicle docking and fusion is mediated by specific interactions between v-SNAREs on vesicles and t-SNAREs on target membranes. GS28 (Golgi SNARE with a size of 28 kDa) is a SNARE that associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi compartments. The majority of GS28, the first 230 and 250 aa, is thought to be anchored to the membrane via the C-terminal hydrophobic tail, which is formed by the remaining 20 aa. GS28 and syntaxin 5, another SNARE, exist as a protein complex in the Golgi and this complex is important for the function of both proteins in ER-Golgi transport. The GS28/syntaxin 5 complex can be dissociated by alpha-SNAP and NSF. In addition, GS28 is thought to interact with alpha-SNAP when the GS28/syntaxin 5 complex is dissociated. Thus, GS28 is a SNARE protein that mediates, in complex with syntaxin 5, transport within the Golgi and between Golgi and ER. This antibody is routinely tested by western blot analysis.
Molecular Weight 28 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Golgi SNAP Receptor Complex Member 1 (GOSR1) (AA 3-108) antibody (ABIN968404) Western blot analysis of GS28 on a RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, la...
 image for anti-Golgi SNAP Receptor Complex Member 1 (GOSR1) (AA 3-108) antibody (ABIN968404) Immunoflourescence of rat neurons.
Product cited in: Loh, Peter, Subramaniam, Hong: "Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus." in: Journal of cell science, Vol. 118, Issue Pt 6, pp. 1209-22, 2005 (PubMed).

Hamza, Prohaska, Gitlin: "Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1215-20, 2003 (PubMed).

Muller, Shorter, Newman, Deinhardt, Sagiv, Elazar, Warren, Shima: "Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion." in: The Journal of cell biology, Vol. 157, Issue 7, pp. 1161-73, 2002 (PubMed).

Lanoix, Ouwendijk, Stark, Szafer, Cassel, Dejgaard, Weiss, Nilsson: "Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1." in: The Journal of cell biology, Vol. 155, Issue 7, pp. 1199-212, 2001 (PubMed).

Subramaniam, Loh, Hong: "N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25441-4, 1997 (PubMed).

Subramaniam, Peter, Philp, Wong, Hong: "GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport." in: Science (New York, N.Y.), Vol. 272, Issue 5265, pp. 1161-3, 1996 (PubMed).